CYP27B1 Gene Polymorphism rs10877012 in Patients Diagnosed with Colorectal Cancer
| Autor: | Elżbieta Kostyra, Maria Latacz, Anna Cieślińska, Roman Grzybowski, Janusz Płomiński, Beata Jarmołowska, Jadwiga Snarska, Ewa Fiedorowicz, Konrad Wroński, Huub F. J. Savelkoul |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
rs10877012 medicine.medical_specialty Colorectal cancer Celbiologie en Immunologie colorectal cancer vitamin D lcsh:TX341-641 Gastroenterology Article 03 medical and health sciences 0302 clinical medicine single nucleotide polymorphism (SNP) Single nucleotide polymorphism (SNP) CYP27B1 Internal medicine medicine Vitamin D and neurology Vitamin D Allele Autocrine signalling Gene Nutrition and Dietetics business.industry Odds ratio medicine.disease 030104 developmental biology Cell Biology and Immunology 030220 oncology & carcinogenesis Rs10877012 WIAS Population study Gene polymorphism business lcsh:Nutrition. Foods and food supply Food Science |
| Zdroj: | Nutrients, Vol 12, Iss 998, p 998 (2020) Nutrients, 12(4) Nutrients 12 (2020) 4 Nutrients Volume 12 Issue 4 |
| ISSN: | 2072-6643 |
| Popis: | Colorectal cancer (CRC) is the third most commonly occurring cancer worldwide. Intestinal cells are CYP27B1 gene expression sites and, as a consequence, they are capable of converting pro-vitamin D into the active paracrine and autocrine forms. It was demonstrated that rs10877012 polymorphism in the CYP27B1 gene influenced the circulating vitamin D level. This provided a rationale for determining the role that this polymorphism plays in the risk of developing colon cancer. In this study, we investigated the association of rs10877012 (T/G) polymorphism in the CYP27B1 gene with CRC susceptibility. The study population (n = 325) included CRC patients (n = 106) and healthy controls (n = 219). DNA was extracted from peripheral leukocytes and analyzed for the CYP27B1 polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. We found an association between the presence of the T allele at the polymorphic site (odds ratio (OR) = 2.94 95% CI 1.77&ndash 4.86 p < 0.0001) and a decreased CRC incidence. |
| Databáze: | OpenAIRE |
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