'Hot spots' of creatine kinase localization in brain: cerebellum, hippocampus and choroid plexus
| Autor: | Wolfram Hemmer, David M. Holtzman, Else Zanolla, Theo Wallimann, Philipp Kaldis |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Gene isoform
Cerebellum Pathology medicine.medical_specialty Fluorescent Antibody Technique Granular layer Isozyme Hippocampus Developmental Neuroscience medicine Animals Electrophoresis Gel Two-Dimensional Tissue Distribution Creatine Kinase biology Cell biology Mitochondria Rats Isoenzymes Cytosol medicine.anatomical_structure nervous system Neurology Microscopy Fluorescence Choroid Plexus biology.protein Creatine kinase Choroid plexus Antibody Chickens |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0378-5866 |
| Popis: | Creatine kinase (CK) isoenzymes, with emphasis on the mitochondrial CK isoenzymes, were characterized and localized in chicken cerebellum. Chicken cerebellar extracts analyzed by two-dimensional gels, using anti-peptide antibodies specific for sarcomeric muscle-type mitochondrial CK (Mib-CK) and revealed the presence of a Mib-CK variant in avian cerebellum. This CK isoform was localized by immunofluorescence staining exclusively in the Purkinje neurons. The co-expression of this Mib-CK together with cytosolic muscle-type MM-CK, as observed in the same Purkinje neurons, may reflect the specific energy requirements associated with highly fluctuating Ca2+ levels (Ca2+ spiking) in these specialized neurons. Ubiquitous brain-type mitochondrial Mia-CK was found together with cytosolic BB-CK mainly in the glomeruli structures of the cerebellar granular layer. BB-CK, but much less so Mia-CK, however, was also very prominent in Bergmann glial cells of the molecular layer. Thus, an isoenzyme-specific differential localization of the two mitochondrial Mi-CK isoenzymes in the chicken cerebellum is demonstrated. Other hot spots of CK localization were the granule and pyramidal cells of the hippocampus in rat. There, a developmental stage-dependent immunofluorescence staining, especially with antibodies against Mia-CK, was noted. Epithelial cells of the choroid plexus were also highly enriched in CK. The possible implications of a CK/PCr circuit at these various cellular locations of the brain are discussed with respect to normal brain physiology and pathology. |
| Databáze: | OpenAIRE |
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