Effects of dietary supplementation with glutamine on the lymphocyte proliferation and intestinal immune gene expression in broiler chickens infected with Salmonella Enteritidis
| Autor: | Dou Dou Wang, Qiu Jue Wu, Zhi Bin Zhang, Ao Ren, Dou Dou Zhu, Bing Bing Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Salmonella Infections
Animal General Veterinary Lymphocyte Salmonella enteritidis Glutamine Broiler Gene Expression Spleen Lymphocyte proliferation Biology Animal Feed Microbiology Diet Basal (phylogenetics) Immune system medicine.anatomical_structure Dietary Supplements medicine Animals Lymphocyte Count Chickens Poultry Diseases Cell Proliferation |
| Zdroj: | Research in veterinary science. 139 |
| ISSN: | 1532-2661 |
| Popis: | An experiment was conducted to investigate the effects of glutamine (Gln) on the lymphocyte proliferation and intestinal immune relevant gene expression in broilers infected with Salmonella Enteritidis. 240 1-day-old broilers were divided randomly into four groups in a completely randomized design, each of which had 6 replicates. Birds were reared in battery cages for 21 days. The experimental groups were as follows: control group (unchallenged group, CON), basal diet; Salmonella Enteritidis challenged group (challenged with 2.0 × 104 CFU/mL of Salmonella Enteritidis, SCC), basal diet; Gln 1, basal diet plus Salmonella Enteritidis challenged plus Gln at 0.5% diet; Gln 2, basal diet plus Salmonella Enteritidis challenged plus Gln at 1.0% diet. The results showed that Salmonella Enteritidis infection led to some decrease in the relative weight of spleen and bursa (except at 21 d), lymphocyte percentage, number of proliferation peripheral blood T and B lymphocytes, and increased the heterophil percentage, H/L ratio, mRNA expression levels of TNF-α, NF-κB p65, IL-1β, IL-6, and IL-8 in the jejunal and ileal mucosa compared with the measurements of these parameters in the CON group at d 4, 7, 14, and 21 (p |
| Databáze: | OpenAIRE |
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