REN1Is Required for Development of Microconidia and Macroconidia, but Not of Chlamydospores, in the Plant Pathogenic FungusFusarium oxysporum
| Autor: | Takashi Tsuge, Hitoshi Kunoh, Toshiaki Ohara, Iori Inoue, Fumio Namiki |
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| Rok vydání: | 2004 |
| Předmět: |
Hypha
Recombinant Fusion Proteins Genes Fungal Molecular Sequence Data Conidiation Biology Microbiology Conidium Fungal Proteins Chlamydospore Open Reading Frames Fusarium Fusarium oxysporum Genetics Amino Acid Sequence DNA Fungal Fungal protein Base Sequence Sequence Homology Amino Acid fungi Fungal genetics food and beverages Plants Spores Fungal Chlamydospore formation biology.organism_classification Mutation Research Article |
| Zdroj: | Genetics. 166:113-124 |
| ISSN: | 1943-2631 |
| Popis: | The filamentous fungus Fusarium oxysporum is a soil-borne facultative parasite that causes economically important losses in a wide variety of crops. F. oxysporum exhibits filamentous growth on agar media and undergoes asexual development producing three kinds of spores: microconidia, macroconidia, and chlamydospores. Ellipsoidal microconidia and falcate macroconidia are formed from phialides by basipetal division; globose chlamydospores with thick walls are formed acrogenously from hyphae or by the modification of hyphal cells. Here we describe rensa, a conidiation mutant of F. oxysporum, obtained by restriction-enzyme-mediated integration mutagenesis. Molecular analysis of rensa identified the affected gene, REN1, which encodes a protein with similarity to MedA of Aspergillus nidulans and Acr1 of Magnaporthe grisea. MedA and Acr1 are presumed transcription regulators involved in conidiogenesis in these fungi. The rensa mutant and REN1-targeted strains lack normal conidiophores and phialides and form rod-shaped, conidium-like cells directly from hyphae by acropetal division. These mutants, however, exhibit normal vegetative growth and chlamydospore formation. Nuclear localization of Ren1 was verified using strains expressing the Ren1-green fluorescent protein fusions. These data strongly suggest that REN1 encodes a transcription regulator required for the correct differentiation of conidiogenesis cells for development of microconidia and macroconidia in F. oxysporum. |
| Databáze: | OpenAIRE |
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