Impact of substrates and cell immobilization on siderophore activity by Pseudomonads in a Fe and/or Cr, Hg, Pb containing-medium
| Autor: | Karine Jézéquel, Armelle Braud, Thierry Lebeau |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Glycerol
Sucrose Bioaugmentation Siderophore Environmental Engineering Health Toxicology and Mutagenesis Microorganism Siderophores Lactose Pseudomonas fluorescens Fructose Microbiology Metal Bioremediation Metals Heavy medicine Animals Soil Pollutants Environmental Chemistry Mannitol Waste Management and Disposal biology Chemistry Pseudomonas Starch biology.organism_classification Pollution Glucose Milk visual_art Pseudomonas aeruginosa visual_art.visual_art_medium Nuclear chemistry medicine.drug |
| Zdroj: | Journal of Hazardous Materials. 144:229-239 |
| ISSN: | 0304-3894 |
| DOI: | 10.1016/j.jhazmat.2006.10.014 |
| Popis: | To increase the amount of bioavailable metals in phytoextraction purposes, soil bioaugmentation with Pseudomonads, as siderophore producers with high metal complexation levels, could be relevant. Unfortunately, siderophore synthesis may be inhibited by soluble iron in soil and bacteria can suffer at the same time from the toxicity of some other metals, predation and oligotrophy. To overcome these drawbacks, we attempted to co-locate a carbon substrate and Pseudomonas aeruginosa or P. fluorescens in Ca-alginate beads. First, free-cell cultures showed that glycerol, fructose, mannitol and skim milk enhanced the siderophore activity which was the highest in the medium with neither Fe or TM (toxic metal) (Cr, Hg and Pb) and the lowest in the Fe-containing medium without TM. The negative effect of iron was partly offset when TM was added to the medium. In a second part, co-location of microorganisms and substrates was only feasible with skim milk. By comparison with free cells, siderophore activity by immobilized cells was higher in culture media containing Fe with or without TM (up to a ratio of 9), and varied in a narrow margin, according to the medium composition. |
| Databáze: | OpenAIRE |
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