High-performance liquid chromatography with evaporative light-scattering detection for the determination of phospholipid classes in human milk, infant formulas and phospholipid sources of long-chain polyunsaturated fatty acids
| Autor: | A Sala Vila, M.C. López-Sabater, M Rodrı́guez-Palmero-Seuma, A.I Castellote-Bargalló |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Light
Phospholipid Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Chromatography detector Phosphatidylcholine Humans Scattering Radiation Chromatography High Pressure Liquid Phospholipids Phosphatidylethanolamine chemistry.chemical_classification Detection limit Chromatography Milk Human Chemistry Organic Chemistry Infant General Medicine Infant formula Fatty Acids Unsaturated lipids (amino acids peptides and proteins) Infant Food Polyunsaturated fatty acid |
| Zdroj: | Journal of chromatography. A. 1008(1) |
| ISSN: | 0021-9673 |
| Popis: | We developed and validated a new high-performance liquid chromatographic method for the separation of phospholipid classes in human milk, infant formulas and phospholipidic sources of long-chain polyunsaturated fatty acids (LC-PUFAs) used in paediatric nutrition. Phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and sphingomyelin were separated in less than 25 min using an Extrasil silica column (150 x 4.0 mm I.D., 3-microm particle size) by isocratic elution with a mixture of isopropanol-hexane-water. Phospholipids were determined by an evaporative light-scattering detector. Several chromatographic conditions were assayed to optimise the method, whose suitability is shown by the detection limits, linearity ranges and precision rates obtained. The main advantages of the proposed method are its speed and the direct determination of the main phospholipids present in human milk, infant formulas and the phospholipid sources of LC-PUFAs used in paediatric nutrition. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect