Interferons antagonize gamma-ray-induced depression of natural immunity
| Autor: | G Starace, Tricarico M, R Pepponi, M. P. Fuggetta, Enzo Bonmassar |
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| Rok vydání: | 1998 |
| Předmět: |
Cancer Research
Lymphocyte Pharmacology Peripheral blood mononuclear cell Natural killer cell Interferon-gamma Immune system In vivo Interferon medicine Humans Radiology Nuclear Medicine and imaging Radiation business.industry Interferon-beta In vitro Immunity Innate Killer Cells Natural medicine.anatomical_structure Oncology Gamma Rays Immunology Interferons business medicine.drug K562 cells |
| Zdroj: | International journal of radiation oncology, biology, physics. 40(4) |
| ISSN: | 0360-3016 |
| Popis: | Purpose: The aim of this study was to determine the inhibitory effects of in vitro radiation on the number and function of natural killer (NK) cells and to investigate the capability of interferons (IFNs) to restore the activity of NK, depressed by γ-rays. Methods and Materials: Mononuclear cells (MNC) were obtained from intact or in vitro irradiated (20 Gy) peripheral blood collected from healthy donors. Alternatively, MNC were irradiated (20 Gy) after separation from intact whole blood. The in vitro treatment of MNC with IFNs (α, β, or γ, 200 UI/ml) was performed at different times after or before radiation. The NK activity (4 h- 51 Cr release test), the percentage of CD16 + /CD56 + cells and apoptosis (cytometric analysis), and binding (microscopic observation) were evaluated on Days 0, 1, 2, and 5 from γ-ray exposure and IFNs treatment. Results: The in vitro treatment of irradiated MNC with βIFN after radiation completely reverses the inhibitory effects of γ-rays on human NK activity. βIFN do not reduce the apoptosis induction by radiation and don't modify the number of CD16- or CD56-positive cells. The binding between irradiated effectors and tumor cells (K562) appears partially increased in βIFN-treated MNC. Conclusions: The results of the present investigation suggest a possible role of βIFN in reversing the detrimental effect of radiation on human natural immunity and provide a rational basis for in vivo use of βIFN in cancer radiotherapy. |
| Databáze: | OpenAIRE |
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