Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction
| Autor: | Boudewijn P.T. Kruithof, Bin Zhou, Lambertus J. Wisse, Marie-José Goumans, Adriana C. Gittenberger-de Groot, Robert E. Poelmann, Michelle D. Tallquist, Sjoerd N. Duim, Roderick W.C. Scherptong, Monique R.M. Jongbloed, William T. Pu, Martin J. Schalij, Rebecca Vicente-Steijn |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Heart Ventricles Myocytes Smooth Muscle Cell Population lcsh:Medicine Biology Both ventricles Mice Internal medicine Basic Helix-Loop-Helix Transcription Factors medicine Animals Myocyte lcsh:Science WT1 Proteins education education.field_of_study Multidisciplinary Myocardium lcsh:R Cardiac Ventricle Embryo Fibroblasts Repressor Proteins medicine.anatomical_structure Ventricle cardiovascular system Cardiology lcsh:Q Regional differences Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 9, p e0136025 (2015) |
| Popis: | Background Morphological and functional differences of the right and left ventricle are apparent in the adult human heart. A differential contribution of cardiac fibroblasts and smooth muscle cells (populations of epicardium-derived cells) to each ventricle may account for part of the morphological-functional disparity. Here we studied the relation between epicardial derivatives and the development of compact ventricular myocardium. Results Wildtype and Wt1CreERT2/+ reporter mice were used to study WT-1 expressing cells, and Tcf21lacZ/+ reporter mice and PDGFRα-/-;Tcf21LacZ/+ mice to study the formation of the cardiac fibroblast population. After covering the heart, intramyocardial WT-1+ cells were first observed at the inner curvature, the right ventricular postero-lateral wall and left ventricular apical wall. Later, WT-1+ cells were present in the walls of both ventricles, but significantly more pronounced in the left ventricle. Tcf21-LacZ + cells followed the same distribution pattern as WT-1+ cells but at later stages, indicating a timing difference between these cell populations. Within the right ventricle, WT-1+ and Tcf21-lacZ+ cell distribution was more pronounced in the posterior inlet part. A gradual increase in myocardial wall thickness was observed early in the left ventricle and at later stages in the right ventricle. PDGFRα-/-;Tcf21LacZ/+ mice showed deficient epicardium, diminished number of Tcf21-LacZ + cells and reduced ventricular compaction. Conclusions During normal heart development, spatio-temporal differences in contribution of WT-1 and Tcf21-LacZ + cells to right versus left ventricular myocardium occur parallel to myocardial thickening. These findings may relate to lateralized differences in ventricular (patho)morphology in humans. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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