Evaluation of immunogenicity and protection of the Mic1-3 knockout Toxoplasma gondii live attenuated strain in the feline host
| Autor: | Chunlei Su, Déborah Voisin, Yohan Villain, Radu Blaga, Isabelle Vallée, Sébastien Perrot, Solen Morisse, Vitomir Djokic, Anne-Claire Lagrée, Edouard Seche, Fanny Boursin, Delphine Le Roux, Vanessa Doré, Aurélie Grasset-Chevillot, Clément Chauvin |
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| Přispěvatelé: | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), VitamFero, Partenaires INRAE, École nationale vétérinaire - Alfort (ENVA), The University of Tennessee [Knoxville], Oséo/BPIFrance (n°A1206018Q), Scientific council of EnvA, Campus France, ANSES, École nationale vétérinaire d'Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, École nationale vétérinaire d'Alfort (ENVA) |
| Rok vydání: | 2019 |
| Předmět: |
Protozoan Vaccines
Definitive host 030231 tropical medicine Population Cat Oocysts Biology Cat Diseases 03 medical and health sciences Feces Gene Knockout Techniques 0302 clinical medicine Immune system Immunogenicity Vaccine parasitic diseases medicine Animals 030212 general & internal medicine Immune response education MESH: Gene Knockout Techniques education.field_of_study MESH: Toxoplasmosis Animal General Veterinary General Immunology and Microbiology T. gondii MESH: Cat Diseases MESH: Protozoan Vaccines Immunogenicity MESH: Toxoplasma Public Health Environmental and Occupational Health Antibody titer Oocysts Toxoplasma gondii MESH: Immunogenicity Vaccine medicine.disease biology.organism_classification Virology Toxoplasmosis 3. Good health Vaccination Titer Infectious Diseases [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Toxoplasmosis Animal Cats Molecular Medicine Mic1-3KO vaccine candidate strain Toxoplasma |
| Zdroj: | Vaccine Vaccine, Elsevier, 2020, 38 (6), pp.1457-1466. ⟨10.1016/j.vaccine.2019.11.076⟩ |
| ISSN: | 1873-2518 0264-410X |
| DOI: | 10.1016/j.vaccine.2019.11.076⟩ |
| Popis: | International audience; Toxoplasmosis is a zoonotic disease caused by the parasite Toxoplasma gondii. Up to a third of the global human population is estimated to carry a T. gondii infection, which can result in severe complications in immunocompromised individuals and pregnant women. Humans and animals can become infected by ingesting either tissue cysts containing T. gondii bradyzoites, from raw or undercooked meat, or sporulated oocysts from environmental sources. T. gondii oocysts are released in the faeces of cats and other felids, which are the parasite's definitive hosts, leading to environmental contamination. Therefore, vaccination of the feline host against T. gondii is an interesting strategy to interrupt the parasitic life cycle and subsequently limit contamination of intermediate hosts. With this goal in mind, we tested in cats, an attenuated live strain of T. gondii deleted for the Mic1 and Mic3 genes (Mic1-3KO) that was previously shown to be an efficient vaccine candidate in mouse and sheep models. Subcutaneous or oral vaccination routes induced a high specific antibody titer in the cat sera, indicating that the Mic1-3KO strain is immunogenic for cats. To assess protection induced by the vaccine candidate strain, we followed oocysts shedding by vaccinated cats, after oral challenge with a T. gondii wild-type strain. Surprisingly, a high antibody titer did not prevent cats from shedding oocysts from the challenge strain, regardless of the vaccination route. Our results show that the Mic1-3KO vaccine candidate is immunogenic in the feline host, is well tolerated and safe, but does not confer protection against oocysts shedding after natural infection with wild type T. gondii. This result highlights the particular relationship between T. gondii and its unique definitive host, which indicates the need for further investigations to improve vaccination strategies to limit environmental and livestock contaminations. |
| Databáze: | OpenAIRE |
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