Enhancing thermophilic dark fermentative hydrogen production at high glucose concentrations via bioaugmentation with Thermotoga neapolitana
| Autor: | Onyinye Okonkwo, Giovanni Esposito, Stefano Papirio, Aino-Maija Lakaniemi, Eric Trably, Renaud Escudié |
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| Přispěvatelé: | University of Tampere [Finland], Università degli studi di Napoli Federico II, Laboratoire de Biotechnologie de l'Environnement [Narbonne] (LBE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Marie Skłodowska-Curie European Joint Doctorate (EJD), European Project: 643071,H2020,H2020-MSCA-ITN-2014,ABWET(2015), Tampere University, Research group: Bio- and Circular Economy, Materials Science and Environmental Engineering, Okonkwo, Onyinye, Papirio, Stefano, Trably, Eric, Escudie, Renaud, Lakaniemi, Aino-Maija, Esposito, Giovanni, University of Naples Federico II = Università degli studi di Napoli Federico II, Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro) |
| Rok vydání: | 2020 |
| Předmět: |
Bioaugmentation
Biohydrogen Dark fermentation Metabolic pathways Microbial dynamics 116 Chemical sciences Energy Engineering and Power Technology Continuous stirred-tank reactor 02 engineering and technology 010402 general chemistry 01 natural sciences Hyda Food science biology [SDE.IE]Environmental Sciences/Environmental Engineering Renewable Energy Sustainability and the Environment Chemistry Thermophile Microbial dynamics 021001 nanoscience & nanotechnology Condensed Matter Physics Thermotoga biology.organism_classification 0104 chemical sciences Fuel Technology Metabolic pathways Yield (chemistry) Fermentative hydrogen production Biohydrogen 0210 nano-technology Thermotoga neapolitana Dark fermentation |
| Zdroj: | International Journal of Hydrogen Energy International Journal of Hydrogen Energy, Elsevier, 2020, 45 (35), pp.17241-17249. ⟨10.1016/j.ijhydene.2020.04.231⟩ International Journal of Hydrogen Energy, 2020, 45 (35), pp.17241-17249. ⟨10.1016/j.ijhydene.2020.04.231⟩ |
| ISSN: | 0360-3199 |
| DOI: | 10.1016/j.ijhydene.2020.04.231 |
| Popis: | The aim of the present study was to investigate the effect of gradually increasing glucose concentrations (from 5.6 to 111 mmol L−1) on the fermentative H2 production with and without bioaugmentation. A stirred tank reactor (STR) was operated at 70 °C and inoculated with a hyperthermophilic mixed culture or a hyperthermophilic mixed culture bioaugmented with Thermotoga neapolitana. With both the unaugmented (control) and augmented cultures, the H2 production rate was improved when the initial glucose concentration was increased. In contrast, the highest H2 yield (1.68 mol H2 mol−1 glucose consumed) was obtained with the augmented culture at the lowest glucose concentration of 5.6 mmol L−1 and was 37.5% higher than that obtained with the unaugmented culture at the same feed glucose concentration. Overall, H2 production rates and yields were higher in the bioaugmented cultures than in the unaugmented cultures whatever the glucose concentration. Quantitative polymerase chain reaction targeting T. neapolitana hydA gene and MiSeq sequencing proved that Thermotoga was not only present in the augmented cultures but also the most abundant at the highest glucose concentrations. acceptedVersion |
| Databáze: | OpenAIRE |
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