USE OF BUTTER MILK AND POULTRY-TRANSFORMING WASTES FOR ENHANCED PRODUCTION OF Bacillus subtilis SPB1 BIOSURFACTANT IN SUBMERGED FERMENTATION
| Autor: | Raida Zouari, Dhouha Ghribi, Semia Ellouze-Chaabouni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Optimization
biology business.industry Chemistry lcsh:QR1-502 food and beverages Biosurfactant Bacillus subtilis Raw material biology.organism_classification Box–Behnken Design Microbiology Submerged fermentation Box–Behnken design lcsh:Microbiology Biotechnology Animal waste Fermentation Food science business Molecular Biology Food Science |
| Zdroj: | Journal of Microbiology, Biotechnology and Food Sciences, Vol vol. 4, Iss no. 5, Pp 462-466 (2015) |
| ISSN: | 1338-5178 |
| Popis: | Biosurfactants are valuable microbial amphiphilic molecules with effective surface-active and biological properties applicable to several industries and processes. Microorganisms synthesize them, especially during growth on water-immiscible substrates, providing an alternative to chemically prepared conventional surfactants. Microbial surfactants are not yet a sustainable alternative to chemically synthesized surfactants seeing their potentially high production charges. This study highlights the use of low-cost agro-industrial raw material for fermentative production of biosurfactants. The Box–Behnken Design and response surface methodology were employed to optimize the concentrations of the ratio butter milk /distilled water, poultry-transforming wastes and inoculum size for lipopeptide biosurfactant production by B.subtilis SPB1 in submerged fermentation.The best production yield was about 12.61 ± 0.7 g/L of crude lipopeptide biosurfactant. It can be obtained when using a ratio butter milk /distilled water of 1.5, poultry-transforming wastes of 23g/L and an inoculum size of 0.12. In comparison to the highest biosurfactant production yield reported for Bacillus subtilis SPB1, three fold increases were obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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