Urocortin 2 and urocortin 3 are expressed by the human placenta, deciduas, and fetal membranes
| Autor: | Pasquale Florio, Enrico Picciolini, Paulo B. Torres, Alberto Imperatore, Paolo Toti, Felice Petraglia, Letizia Galleri, Wylie Vale, Rossella Occhini, Michela Torricelli |
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| Rok vydání: | 2005 |
| Předmět: |
endocrine system
medicine.medical_specialty Urocortin 2 Corticotropin-Releasing Hormone Urocortin 3 Placenta Extraembryonic Membranes Biology Neuropeptides Pregnancy Trophoblast Syncytiotrophoblast Adrenocorticotropic Hormone Fetal membrane Internal medicine medicine Decidua Humans RNA Messenger Cellular localization Urocortins Urocortin Fetus Cytotrophoblast Obstetrics and Gynecology Immunohistochemistry Trophoblasts Pregnancy Trimester First medicine.anatomical_structure Endocrinology Pregnancy Trimester Second embryonic structures Female Endothelium Vascular Chorionic Villi hormones hormone substitutes and hormone antagonists |
| Zdroj: | American journal of obstetrics and gynecology. 195(1) |
| ISSN: | 1097-6868 |
| Popis: | Objective Urocortin 2 (UCN2) and urocortin 3 (UCN 3) are recently identified neuropeptides showing homology to corticotropin-releasing factor (CRF). In the present study, we evaluated their expression and localization in gestational tissues (placenta, decidua, fetal membranes), and their effect on placental adrenocorticotropic hormone secretion. Study design The study was performed in a tertiary clinical care center. Tissues were obtained at first (n = 8; 8-11 weeks of pregnancy) and third (n = 8; 38-40 gestational weeks) trimester. The mRNA expression was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR); the cellular localization by immunohistochemistry; ACTH levels were measured in media collected from cultured placental villi. Results All tissues analyzed expressed UCN2 and UCN3 mRNA. UCN2 and UCN3 were localized in cytotrophoblast and syncytiotrophoblast cells; UCN2 was present in maternal and fetal vessels and in amniotic cells, while UCN3 was absent. Finally, UCN2 and UCN3 did not stimulate ACTH secretion. Conclusion Gestational tissues differentially express UCN2 and UCN3 and, despite their homology to CRF, they did not stimulate placental ACTH secretion. |
| Databáze: | OpenAIRE |
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