Allergen immunotherapy induces a suppressive memory response mediated by IL-10 in a mouse asthma model
| Autor: | Joost Lambert Max Vissers, Frank Reinoud Weller, Antoon J.M. Van Oosterhout, Martien L. Kapsenberg, Betty C.A.M. van Esch, Gerard A. Hofman |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Cell Biology and Histology |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Male
Allergy medicine.medical_treatment Immunoglobulin E LYMPHOCYTES regulatory T cells Immune tolerance Mice hyper-responsiveness Immunology and Allergy Lung T(H)2 lymphocytes Mice Inbred BALB C biology INDUCTION MURINE MODEL respiratory system suppression Interleukin-10 Interleukin 10 Cytokine IL-10 Cytokines immunotherapy IgE Bronchoalveolar Lavage Fluid eosinophilia Allergen immunotherapy Ovalbumin Immunology ANTIGEN AIRWAY EOSINOPHILIA HYPERRESPONSIVENESS Immune Tolerance medicine Animals business.industry GRASS-POLLEN IMMUNOTHERAPY Immunotherapy asthma GAMMA medicine.disease allergy respiratory tract diseases Desensitization Immunologic biology.protein T-CELLS Lymph Nodes Interleukin-5 business Immunologic Memory |
| Zdroj: | Journal of Allergy and Clinical Immunology, 113(6), 1204-1210. MOSBY-ELSEVIER Journal of allergy and clinical immunology, 113(6), 1204-1210. Mosby Inc. |
| ISSN: | 0091-6749 |
| Popis: | Background Human studies have demonstrated that allergen immunotherapy induces memory suppressive responses and IL-10 production by allergen-specific T cells. Previously, we established a mouse model in which allergen immunotherapy was effective in the suppression of allergen-induced asthma manifestations. Objective In this study, we examined whether immunotherapy induces a long-lasting effect and investigated the role of IL-10 in successful immunotherapy. Methods Ovalbumin-sensitized BALB/c mice were treated with 3 injections of ovalbumin (1 mg, subcutaneous) on alternate days. After a short interval (1 week) and after a long interval (5 weeks), mice were challenged by ovalbumin inhalation, and subsequently, airway reactivity, airway eosinophilia, ovalbumin-specific IgE, and T H 2 cytokine profile were measured. Flow cytometry and blocking of IL-10 receptors in vivo were used to gain insight in the role of IL-10 in the beneficial effects of allergen immunotherapy. Results After a long interval between ovalbumin immunotherapy and ovalbumin challenge, the development of airway eosinophilia and hyperresponsiveness to methacholine were as strongly suppressed as after a short interval. These suppressive effects coincided with significantly reduced serum ovalbumin-specific IgE levels and T H 2 cytokine production. On immunotherapy, the IL-5:IL-10 ratio in the bronchoalveolar lavage fluid shifted toward IL-10. In ovalbumin-restimulated lung cell and thoracic lymph node cultures from these mice, IL-5 levels dramatically decreased, whereas the percentage of IL-10 + CD4 + T cells was not affected. Finally, in mice treated with mAb against IL-10 receptors, the beneficial effects of immunotherapy were largely abrogated. Conclusion These data demonstrate that allergen immunotherapy induces a memory suppressive effect in which IL-10 is essential. |
| Databáze: | OpenAIRE |
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