| Autor: |
Elham Ettehadieh, Arvia E. Morris, Teri L. Aldrich, Sharon T. Wong-Madden, Kelly Lane |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Animal Cell Technology: From Target to Market ISBN: 9789401038973 |
| Popis: |
As more genes are being identified through genomic techniques,the need to rapidly express recombinant proteins for functionalstudies has become increasingly acute. Transient expression ofrecombinant protein using COS-1, CV-1 and 293 cells is widelyused to address this need. To improve the robustness of hostcells for transient expression, the effect of over-expression ofProtein Kinase Balpha has been explored. In this report wedemonstrate that over-expression of Protein Kinase Balpha canimprove transient recombinant protein expression 40% to200%depending on the protein being expressed and the cell line used. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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