Multiplexed aberration measurement for deep tissue imaging in vivo
| Autor: | Douglas S. Kim, Rui Liu, Zhongchao Tan, Daniel E. Milkie, Tsai Wen Chen, Chen Wang, Na Ji, Wenzhi Sun, Aaron M. Kerlin |
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| Rok vydání: | 2014 |
| Předmět: |
Fluorescence-lifetime imaging microscopy
Optics and Photonics Light Phase (waves) Neuroimaging Biochemistry Article Histones Mice Optics Microscopy Animals Adaptive optics Caenorhabditis elegans Molecular Biology Zebrafish Fluorescent Dyes Visual Cortex Physics Fourier Analysis business.industry Scattering Resolution (electron density) Brain Proteins Pupil Cell Biology Ray Functional imaging Mice Inbred C57BL Microscopy Fluorescence business Protein Processing Post-Translational Biotechnology |
| Zdroj: | Nature methods |
| ISSN: | 1548-7105 |
| Popis: | We describe a multiplexed aberration measurement method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine their phase gradients. Applicable to fluorescent-protein-labeled structures of arbitrary complexity, it allows us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improves structural and functional imaging of fine neuronal processes over a large imaging volume. |
| Databáze: | OpenAIRE |
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