Individual monitoring of immune responses in rainbow trout after cohabitation and intraperitoneal injection challenge with Yersinia ruckeri
| Autor: | Christopher J. Secombes, Bertrand Collet, Milena Mira Monte, Katy Urquhart |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Fish Proteins
Yersinia ruckeri 0301 basic medicine Yersinia Infections medicine.medical_treatment Antimicrobial peptides Intraperitoneal injection Adaptive Immunity Aquatic Science Biology Microbiology Fish Diseases 03 medical and health sciences Immune system medicine Animals Environmental Chemistry Pathogen 04 agricultural and veterinary sciences General Medicine biology.organism_classification Acquired immune system Immunity Innate 030104 developmental biology Oncorhynchus mykiss Host-Pathogen Interactions Immunology 040102 fisheries 0401 agriculture forestry and fisheries Rainbow trout Injections Intraperitoneal Antimicrobial Cationic Peptides |
| Zdroj: | Fish & Shellfish Immunology. 55:469-478 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2016.05.041 |
| Popis: | Yersinia ruckeri, the causative agent of enteric red mouth disease (ERM), is a widely studied pathogen in disease models using rainbow trout. This infection model, mostly based on intraperitoneally injection or bath immersion challenges, has an impact on both components (innate and adaptive) of the fish immune system. Although there has been much attention in studying its host-pathogen interactions, there is still a lack of knowledge regarding the impact of a cohabitation challenge. To tackle this we used a newly established non-lethal sampling method (by withdrawing a small amount of blood) in rainbow trout which allowed the individual immune monitoring before (non-infected) and after infection with Yersinia ruckeri either by intraperitoneal (i.p.) injection or by cohabitation (cohab). A range of key immune genes were monitored during the infection by real-time PCR, and results were compared between the two infection routes. Results indicated that inflammatory (IL-1β1 and IL-8) cytokines and certain antimicrobial peptides (cathelicidins) revealed a different pattern of expression between the two infected groups (i.p. vs cohab), in comparison to adaptive immune cytokines (IL-22, IFN-γ and IL-4/13A) and β-defensins. This suggests a different involvement of distinct immune markers according to the infection model, and the importance of using a cohabitation challenge as a more natural disease model that likely simulates what would occur in the environment. |
| Databáze: | OpenAIRE |
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