Cytotoxicity Study of Cyclopentapeptide Analogues of Marine Natural Product Galaxamide towards Human Breast Cancer Cells
| Autor: | Xiao-Jian Liao, Poonam Bhadja, Jignesh Lunagariya, Shi-Hai Xu, Weili Long, Shenghui Zhong, Hangbin Li, Bing-Xin Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Aging Article Subject Breast Neoplasms Biochemistry Peptides Cyclic HeLa 03 medical and health sciences Breast cancer medicine Humans MTT assay lcsh:QH573-671 Cytotoxicity skin and connective tissue diseases Biological Products biology Chemistry lcsh:Cytology Cancer Cell Biology General Medicine Cell cycle medicine.disease biology.organism_classification 030104 developmental biology Cancer cell Cancer research Female Breast carcinoma Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity Oxidative Medicine and Cellular Longevity, Vol 2017 (2017) |
| ISSN: | 1942-0900 |
| DOI: | 10.1155/2017/8392035 |
| Popis: | Herein, we report the cytotoxicity of cyclopentapeptide analogues of marine natural product galaxamide towards breast carcinoma cells and the underlying mechanisms. We examined the effect of the novel galaxamide analogues on cancer cell proliferation by MTT assay and also further examined the most active compound for morphological changes using Hoechst33342 staining technique, induction of apoptosis, cell cycle phases, mitochondrial membrane potential (MMP), and reactive oxygen species (ROS) generation using flow cytometry in human breast cancer MCF-7 cells in vitro. Galaxamide and its analogues effectively induced toxicity in human hepatocellular carcinoma HepG2, human breast carcinoma MCF-7, human epitheloid cervix carcinoma HeLa, and human breast carcinoma MB-MDA-231 cell lines. Amongst them, compound 3 exhibited excellent toxicity towards MCF-7 cells. This galaxamide analogue significantly induced apoptosis in a dose-dependent manner in MCF-7 cells involves cell cycle arrest in the G1 phase, a reduction of MMP, and a marked increase in generation of ROS. Particularly, compound 3 of galaxamide analogues might be a potential candidate for the treatment of breast cancer. |
| Databáze: | OpenAIRE |
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