Development and scale-up of a fed-batch process for the production of capsular polysaccharide from Haemophilus influenzae
| Autor: | Joshua Merritt, George Allard, Lawrence O’Toole, Peter Licari, Randall W. Swartz |
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| Rok vydání: | 2000 |
| Předmět: |
Time Factors
Cell Culture Techniques Bioengineering medicine.disease_cause Polysaccharide Applied Microbiology and Biotechnology Microbiology Haemophilus influenzae medicine Bacterial Capsules Haemophilus Vaccines chemistry.chemical_classification biology Polysaccharides Bacterial Pasteurellaceae Sterilization General Medicine biology.organism_classification Titer chemistry Fermentation Maximum Cell Density Bacteria Biotechnology Conjugate |
| Zdroj: | Journal of Biotechnology. 81:189-197 |
| ISSN: | 0168-1656 |
| Popis: | The production of the capsular polysaccharide, polyribosylribitolphosphate, from Haemophilus influenzae type b is important for the production of effective conjugate vaccines. Factors limiting the production of this polysaccharide from H . influenzae type b in liquid culture were investigated. A fed-batch fermentation was developed that increased cell density and PRP titer approximately four fold when compared to the batch fermentation. This fed-batch process was successfully scaled from the 1.5 l development scale to the 500 l manufacturing scale. The maximum cell density in the 500 l fermentation was 6 g dry cell weight per liter and the PRP concentration was 1.3 g l −1 . |
| Databáze: | OpenAIRE |
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