Detection of toxoplasmosis in pets and stray cats through molecular and serological techniques in Khyber Pakhtunkhwa, Pakistan
| Autor: | Sanaullah Khan, Abdul Majid, Shehzad Zareen, Rafiq Hussain, Sohail, Noor Ul Akbar, Sumbal Haleem, Muhammad Taib, Nisar Ahmad |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Veterinary medicine Population Seroprevalence Enzyme-Linked Immunosorbent Assay Biology Cat Diseases Polymerase Chain Reaction Serology Khyber Pakhtunkhwa ELISA PCR Seroepidemiologic Studies SF600-1100 parasitic diseases medicine Animals Parasite hosting Pakistan education Genotyping education.field_of_study CATS T. gondii General Veterinary Research Age Factors Toxoplasma gondii Pets General Medicine medicine.disease biology.organism_classification Toxoplasmosis Toxoplasmosis Animal Cats Female Toxoplasma |
| Zdroj: | BMC Veterinary Research BMC Veterinary Research, Vol 17, Iss 1, Pp 1-7 (2021) |
| ISSN: | 1746-6148 |
| DOI: | 10.1186/s12917-021-03064-9 |
| Popis: | Background Toxoplasma gondii is an important parasite that belongs to the phylum Apicomplexa, distributed globally, causing major health issues for a wide range of hosts, including humans, native and wild animals. Methods In the present study, we detected IgG and IgM antibodies through an ELISA kit and DNA of T. gondii through PCR in 197 pets and stray cats in Peshawar, Charsadda, Mardan, and Kohat districts of Khyber Pakhtunkhwa (Pakistan) to estimate the existence of feline toxoplasmosis. Results The current study revealed that stray cats have a significant infection rate of T. gondii (74.6%) as compared to pet cats (25.4%). In all the four districts, the prevalence of T. gondii was pointedly higher in district Kohat (95.5%) in the feline population. In comparison to the female (75.18%) and male (both pets and stray) cats have a maximum infection of (81.66%) non-significantly. The prevalence of T. gondii was observed to be significantly higher (91.66%) in the older and greater than 4 year old population of cats as compared to the younger ones. In poor health condition, the cat populations has a higher risk of infection of 92.3% as compared to healthy and poor body condition (73.91%) and (82.6%) respectively. The chronic and reactivated chronic conditions of toxoplasmosis were higher (58.37%) as compared to the acute condition. Conclusion It has been concluded that toxoplasmosis is widely spread in the studied population.The outcomes of the present study show that T. gondii infection has a significant impact on the type of cat, age, and area, which implies a serious threat to human beings. Therefore, genotyping of T. gondii strains from different hosts is needed to forecast the current approach for prevention and control of this zoonotic parasite. |
| Databáze: | OpenAIRE |
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