Main components of pomegranate, ellagic acid and luteolin, inhibit metastasis of ovarian cancer by down-regulating MMP2 and MMP9
| Autor: | Lingqin Dong, Yu-Jie Zhou, Sijing Hu, Yingying Qi, Tingting Gao, Wei Liu, Siwen Wang, Ting Li, Shan Gao, Qing-Hai Li, Hong-Xia Bao, Huidi Liu, Hongyue Wang, Miao Yu, Yongfang Liu, Shu-Lin Liu, Randal N. Johnston, Zheng Zeng, Emilio Mastriani, Zhihang Shen, Xiaoyu Wang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research MMP2 endocrine system diseases MMP9 Metastasis Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ellagic Acid Cell Movement Cell Line Tumor medicine Animals Humans Neoplasm Metastasis luteolin pomegranate fruit juice Cell Proliferation Lythraceae Ovarian Neoplasms Pharmacology Cell growth Chemistry Cancer medicine.disease Xenograft Model Antitumor Assays Research Papers Gene Expression Regulation Neoplastic ovarian cancer 030104 developmental biology Matrix Metalloproteinase 9 Oncology 030220 oncology & carcinogenesis Cancer research Matrix Metalloproteinase 2 Molecular Medicine Female Ovarian cancer Luteolin Ellagic acid |
| Zdroj: | Cancer Biology & Therapy |
| ISSN: | 1555-8576 1538-4047 |
| DOI: | 10.1080/15384047.2017.1394542 |
| Popis: | Ovarian cancer is the third most common cancer in the female reproductive organs and epithelial ovarian cancer has the highest lethality of all gynecological cancers. Pomegranate fruit juice (PFJ) has been shown to inhibit the growth of several types of cancer other than ovarian cancer. In this study, we exposed the ovarian cancer cell line A2780 to PFJ and two of its components (ellagic acid and luteolin). MTT and wound healing assays demonstrated that all three treatments suppressed the proliferation and migration of the ovarian cancer cells. In addition, western blotting and ELISA assays showed that the expression levels of MMP2 and MMP9 gradually decreased after treatment with increasing concentrations of ellagic acid and luteolin. To confirm our findings in the in vitro experiments, we used another ovarian cancer cell line, ES-2, in nude mice experiments. All three treatments inhibited tumor growth without obvious side-effects. Furthermore, compared with the control group, the expression levels of MMP2 and MMP9 were depressed. Ellagic acid induced a greater effect than luteolin, suggesting that ellagic acid might be a promising candidate for further preclinical testing for treatment of human ovarian cancer. |
| Databáze: | OpenAIRE |
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