Rapid detection of avian leukosis virus using a fluorescent microsphere immunochromatographic test strip assay
| Autor: | Huanan Wang, Qiwen Wu, Lefeng Wang, Jianchi Guan, Luo Mengzhen, Zizengchen Wang, Xiangnan Liu, Pan Yu, Yue Shi, Zhu Yujun |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
viruses Infectious bronchitis virus Monoclonal antibody medicine.disease_cause Antibodies Viral Newcastle disease Cross-reactivity Virus Infectious bursal disease 03 medical and health sciences medicine Animals Poultry Diseases 030304 developmental biology Immunoassay 0303 health sciences biology Avian Leukosis Virus Chemistry 0402 animal and dairy science Antibodies Monoclonal 04 agricultural and veterinary sciences General Medicine biology.organism_classification medicine.disease 040201 dairy & animal science Virology Microspheres Capsid Avian Leukosis biology.protein Animal Science and Zoology Capsid Proteins Antibody Chickens |
| Zdroj: | Poultry science. 98(12) |
| ISSN: | 1525-3171 |
| Popis: | We developed a rapid fluorescent microsphere immunochromatographic test strip (FM-ICTS) assay for the quantitative detection of avian leukosis virus (ALV). A monoclonal antibody specific for the ALV major capsid protein encoded by the gag gene was coupled to label fluorescent microspheres. ALV antibodies were coated on a nitrocellulose membrane to prepare a test line for sample detection. The fluorescence signals of the test and control lines can be read either visually by exposure to UV light or using a fluorescence analyzer. ALV could be detected quantitatively using the ratio of fluorescence signals of the test and control lines (T/C). The assay threshold was determined as a T/C value of 0.0606. The fitting curve equation was established between 1 and 2,048 ng/mL P27 protein with an r2 value of 0.9998. The assay showed no cross reactivity with Newcastle disease virus, infectious laryngotracheitis virus, infectious bronchitis virus, Marek's disease virus, infectious bursal disease, Reoviridae virus, or avian influenza virus. The repeatability was satisfactory with an overall average CV of 8.65%. The Kappa coefficient between a commercial ELISA kit was 0.7031 using clinical chicken meconium samples. Thus, a simple, rapid, sensitive, and specific fluorescent microsphere immunochromatographic test strip was developed based on specific anti-capsid protein p27 monoclonal antibodies. |
| Databáze: | OpenAIRE |
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