2D MOF-Based Photoelectrochemical Aptasensor for SARS-CoV-2 Spike Glycoprotein Detection
| Autor: | Chun Mei Li, Yuan Fang Li, Cheng Zhi Huang, Tingting Zhao, Zhong Wei Jiang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Materials science
Porphyrins Modified dna Light metal−organic framework Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Aptamer Immobilized Nucleic Acids Metal Nanoparticles Biosensing Techniques plasmon Limit of Detection Humans General Materials Science aptasensor Ytterbium Electrodes Plasmon Metal-Organic Frameworks spike glycoprotein Photocurrent Detection limit chemistry.chemical_classification Base Sequence SARS-CoV-2 COVID-19 DNA Electrochemical Techniques Aptamers Nucleotide Photochemical Processes Combinatorial chemistry chemistry Electrode Spike Glycoprotein Coronavirus Pharynx Gold Glycoprotein photoelectrochemical Research Article |
| Zdroj: | ACS Applied Materials & Interfaces |
| ISSN: | 1944-8252 |
| Popis: | A reliable and sensitive detection approach for SARS-CoV 2 is essential for timely infection diagnosis and transmission prevention. Here, a two-dimensional (2D) metal-organic framework (MOF)-based photoelectrochemical (PEC) aptasensor with high sensitivity and stability for SARS-CoV 2 spike glycoprotein (S protein) detection was developed. The PEC aptasensor was constructed by a plasmon-enhanced photoactive material (namely, Au NPs/Yb-TCPP) with a specific DNA aptamer against S protein. The Au NPs/Yb-TCPP fabricated by in situ growth of Au NPs on the surface of 2D Yb-TCPP nanosheets showed a high electron-hole (e-h) separation efficiency due to the enhancement effect of plasmon, resulting in excellent photoelectric performance. The modified DNA aptamer on the surface of Au NPs/Yb-TCPP can bind with S protein with high selectivity, thus decreasing the photocurrent of the system due to the high steric hindrance and low conductivity of the S protein. The established PEC aptasensor demonstrated a highly sensitive detection for S protein with a linear response range of 0.5-8 µg/mL with a detection limit of 72 ng/mL. This work presented a promising way for the detection of SARS-CoV 2, which may conduce to the impetus of clinic diagnostics. |
| Databáze: | OpenAIRE |
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