Application of dried blood spot specimens for serologic subtyping of human immunodeficiency virus type 1 in Thailand
| Autor: | M. S. de Souza, P. Tienamporn, N. Limpairojn, T. C. VanCott, Rapee Trichavaroj, P. Puthakird, Penprapa Chanbancherd, Arthur Brown |
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| Rok vydání: | 1999 |
| Předmět: |
Microbiology (medical)
Serotype HIV Antigens Molecular Sequence Data V3 loop Biology HIV Antibodies Polymerase Chain Reaction Sensitivity and Specificity Virus Virology medicine Humans Typing Amino Acid Sequence Serotyping Acquired Immunodeficiency Syndrome Blood Specimen Collection medicine.diagnostic_test Reproducibility of Results Thailand Subtyping Dried blood spot surgical procedures operative Immunoassay HIV-1 Nested polymerase chain reaction |
| Zdroj: | Journal of clinical microbiology. 37(3) |
| ISSN: | 0095-1137 |
| Popis: | Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity. |
| Databáze: | OpenAIRE |
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