Delivery of Differentiation Factors by Mesoporous Silica Particles Assists Advanced Differentiation of Transplanted Murine Embryonic Stem Cells
| Autor: | Elena N. Kozlova, Niclas König, Håkan Aldskogius, Chunfang Zhou, Alfonso E. Garcia-Bennett, Richardson N. Leão, Vladimir Berezin, Elisabeth Bock, Mariya Kozhevnikova, Carl Trolle, Thomas Knöpfel, Stanislava Pankratova |
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| Rok vydání: | 2013 |
| Předmět: |
Nervous system
Embryonic stem cells Transplantation Cellular differentiation Cell Biology General Medicine Biology Ciliary neurotrophic factor Embryonic stem cell Neural stem cell Cell biology Neurotrophic factors Differentiation Tissue Engineering and Regenerative Medicine Glial cell line-derived neurotrophic factor biology.protein Cell transplantation Neural differentiation Stem cell Stem cell culture Developmental Biology |
| Zdroj: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
| ISSN: | 2157-6580 2157-6564 |
| DOI: | 10.5966/sctm.2013-0072 |
| Popis: | Stem cell transplantation holds great hope for the replacement of damaged cells in the nervous system. However, poor long-term survival after transplantation and insufficiently robust differentiation of stem cells into specialized cell types in vivo remain major obstacles for clinical application. Here, we report the development of a novel technological approach for the local delivery of exogenous trophic factor mimetics to transplanted cells using specifically designed silica nanoporous particles. We demonstrated that delivering Cintrofin and Gliafin, established peptide mimetics of the ciliary neurotrophic factor and glial cell line-derived neurotrophic factor, respectively, with these particles enabled not only robust functional differentiation of motor neurons from transplanted embryonic stem cells but also their long-term survival in vivo. We propose that the delivery of growth factors by mesoporous nanoparticles is a potentially versatile and widely applicable strategy for efficient differentiation and functional integration of stem cell derivatives upon transplantation. |
| Databáze: | OpenAIRE |
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