New insights about functional and cross-reactive properties of antibodies generated against recombinant TbpBs of Haemophilus parasuis
| Autor: | Bibiana Martins Barasuol, Luiz Carlos Kreutz, Anthony B. Schryvers, Rafael Frandoloso, Elías F. Rodríguez-Ferri, César B. Gutiérrez-Martín, Jamie E. Fegan, João Antônio Guizzo, Sonia Martínez-Martínez |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Serotype Haemophilus Infections Swine medicine.medical_treatment 030106 microbiology lcsh:Medicine Heterologous Cross Reactions Biology Article Epitope Microbiology Transferrin-Binding Protein B Epitopes Haemophilus parasuis 03 medical and health sciences Classical complement pathway Adjuvants Immunologic Antigen medicine Animals lcsh:Science Haemophilus Vaccines Swine Diseases Antiserum Multidisciplinary Virulence lcsh:R Transferrin Antibodies Bacterial Virology Recombinant Proteins 030104 developmental biology biology.protein lcsh:Q Antibody Adjuvant |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-10627-0 |
| Popis: | Vaccines have become fundamental in the control and elimination of Glässer Disease, a systemic disease of pigs caused by Haemophilus parasuis. The classic vaccines available for prevention of this infection were developed without a robust knowledge about host immunological mechanisms. In this study, we demonstrated the presence of cross-reactive epitopes on both the N-lobe and C-lobe of variants of transferrin binding protein B (TbpBs) expressed on the surface of 6 virulent serovars of H. parasuis. Antibodies against TbpB-derived antigens were capable of increasing the phagocytic capacity of neutrophils and were also capable of blocking porcine transferrin from binding to TbpB. Surprisingly, none of the pig or mice antisera from animals immunized with TbpB-derived antigens mixed with Montanide IMS 2215 VG PR adjuvant were able to activate the classical complement pathway (CCP). In contrast, antisera from mice immunized with TbpB-derived antigens adjuvanted with Freund’s adjuvants or Montanide Gel 01 were able to activate the CCP and kill H. parasuis. Our results demonstrate that the type of adjuvant can modulate the functional response induced by TbpB-derived antigens. Based on these results, we propose that a properly formulated TbpB-based vaccine may elicit a functional protective antibody response with broad cross-reactivity against heterologous strains of H. parasuis. |
| Databáze: | OpenAIRE |
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