Molecular-genetic Characterization of Shiga-Toxin Producing Escherichia Coli Isolated During a Food-Borne Outbreak in St. Petersburg in 2013
| Autor: | O N Bushmanova, L. A. Kaftyreva, E. V. Kicha, N N Kartsev, Vasily A. Bannov, Onishchenko Gg, F B Zhebrun, I.A. Dyatlov, I G Shemyakin, O V Polosenko, M. G. Teimurazov, M A Makarova, Vera P. Myakinina, Alexander G. Bogun, Borzenkov Vn, N S Grigor'eva, Nadezhda K. Fursova, Anastasia V. Popova, A V Stalevskaya, Yu. N. Korzhaev, Edward A. Svetoch, G V Zabalueva, T B Kutasova, I G Tchinjeria, N. S. Bashketova, Nikolay V. Volozhantsev, Angelina A. Kislichkina, T. A. Grechaninova, Z. N. Matveeva |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Serotype Disease reservoir Biology medicine.disease_cause Disease Outbreaks Russia Microbiology Foodborne Diseases fluids and secretions STX2 medicine Animals Humans Child Escherichia coli Escherichia coli Infections Prophage Disease Reservoirs Intimin Shiga-Toxigenic Escherichia coli Infant Outbreak General Medicine biochemical phenomena metabolism and nutrition Raw milk Colitis bacterial infections and mycoses Virology Milk Child Preschool Hemolytic-Uremic Syndrome Female Genome-Wide Association Study |
| Zdroj: | Europe PubMed Central Scopus-Elsevier |
| ISSN: | 0869-6047 |
| DOI: | 10.15690/vramn.v70i1.1234 |
| Popis: | Shiga toxin-producing Escherichia coli (STEC) food-borne infections are reported worldwide and represent a serious problem for public healthcare. In the Russian Federation there is little information on epidemiology and etiology of STEC-infections as well as on molecular-genetic peculiarities of STEC pathogens. Objective : Our aim was to describe a food-borne outbreak as hemorrhagic colitis (HC) along with hemolytic uremic syndrome (HUS), enterocolitis, and acute gastroenteritis in children in St.-Petersburg in 2013. Methods : Epidemiological, microbiological, molecular-genetic and bioinformati с methods were applied. Results : Objects to study were clinical specimens, milk and food samples, as well as STEC strains isolated during the outbreak. The outbreak of food-borne infection was found to be caused by STEC-contaminated raw milk as confirmed by epidemiological analysis, detection of STEC DNA and isolation of relevant pathogens in milk and sick children fecal specimens. The whole-genome sequencing revealed two groups of pathogens, E. coli O157:H7 and E. coli О 101:H33 among collected strains. Group I strains were attributed to the previously known sequence type ST24, while group II strains belonged to the previously non-described sequence type ST145. In strain genomes of both groups there were identified nucleotide sequences of VT2-like prophage carrying stx2 с gene, plasmid enterohemolysin gene, and gene of the STEC main adhesion factor intimin. Gene of intimin gamma was identified in E. coli O157:H7 strains and intimin iota 2 in E. coli O101:H33 strains. The latter previously was identified only in enteropathogenic E. coli (EPEC) strains. Conclusion : The additional knowledge of epidemiology and biology of STEC pathogens would assist clinicians and epidemiologists in diagnosing, treating and preventing hemorrhagic colitis. |
| Databáze: | OpenAIRE |
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