Integrative quantitative-phase and airy light-sheet imaging
| Autor: | Demetrios N. Christodoulides, Scott E. Baker, Nava R. Subedi, Shahla Nemati, Andreas E. Vasdekis, Pawel S. Jung, Erin L. Bredeweg |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Multidisciplinary Computer science Light-sheet microscopy Microfluidics lcsh:R Inverted microscope lcsh:Medicine Cellular noise Article Optical imaging Interference microscopy 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Microscopy lcsh:Q Biological system lcsh:Science 030217 neurology & neurosurgery Gaussian beam |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020) |
| ISSN: | 2045-2322 |
| Popis: | Light-sheet microscopy enables considerable speed and phototoxicity gains, while quantitative-phase imaging confers label-free recognition of cells and organelles, and quantifies their number-density that, thermodynamically, is more representative of metabolism than size. Here, we report the fusion of these two imaging modalities onto a standard inverted microscope that retains compatibility with microfluidics and open-source software for image acquisition and processing. An accelerating Airy-beam light-sheet critically enabled imaging areas that were greater by more than one order of magnitude than a Gaussian beam illumination and matched exactly those of quantitative-phase imaging. Using this integrative imaging system, we performed a demonstrative multivariate investigation of live-cells in microfluidics that unmasked that cellular noise can affect the compartmental localization of metabolic reactions. We detail the design, assembly, and performance of the integrative imaging system, and discuss potential applications in biotechnology and evolutionary biology. |
| Databáze: | OpenAIRE |
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