Increasing participation of sclerostin in postnatal bone development, revealed by three-dimensional immunofluorescence morphometry

Autor: Yoshihiro Tamamura, Teruo Amagasa, Takashi Watanabe, Yuji Makino, Tadahiro Iimura, Akira Yamaguchi, Hiroshi Kamioka, Akiyoshi Hoshino
Rok vydání: 2012
Předmět:
Male
Aging
Indoles
Time Factors
Physiology
Endocrinology
Diabetes and Metabolism
Fluorescent Antibody Technique
Cell Count
Core Binding Factor Alpha 1 Subunit
law.invention
Mice
chemistry.chemical_compound
law
Oximes
Femur
Wnt Signaling Pathway
beta Catenin
Periosteum
Microscopy
Confocal
biology
Protein Stability
Chemistry
musculoskeletal
neural
and ocular physiology
Osteoblast
Protein Transport
medicine.anatomical_structure
Sp7 Transcription Factor
Osteocyte
Bone Morphogenetic Proteins
Osteocalcin
Genetic Markers
medicine.medical_specialty
Histology
Osteocytes
Cell Line
Imaging
Three-Dimensional
Confocal microscopy
Internal medicine
medicine
Animals
Cell Nucleus
Bone Development
DMP1
Rats
Endocrinology
Animals
Newborn
biology.protein
Sclerostin
Cortical bone
Diaphyses
Transcription Factors
Zdroj: Bone. 51:447-458
ISSN: 8756-3282
DOI: 10.1016/j.bone.2012.06.019
Popis: Confocal immunofluorescence tiling imaging revealed the spatio-temporal distributions of osterix and sclerostin in femurs from 3-day-old, 2-week-old and 4-week-old rats to be reciprocally exclusive at the tissue level. Further quantitative three-dimensional immuno fluorescence morphometry demonstrated the increasing distribution of sclerostin in the osteocytic lacuno-canalicular system specifically in diaphysis, which paralleled the cooperative participation and depletion of osterix and β-catenin in adjacent periosteum cells. Treating MC3T3-E1 cells with BIO (a GSK3 inhibitor) induced the stabilization of β-catenin and nuclear translocation of osterix, and negatively regulated osteocalcin/BGLAP and Dmp1. These results collectively demonstrate that the increasing distribution of sclerostin in diaphyseal cortical bone appears to be involved in the attenuation of osterix and β-catenin in adjacent periosteum cells, thus possibly contributing to osteoblast maturation and reducing the osteoblast formation at this bone site. Our confocal microscopy-based imaging analyses provide a comprehensive and detailed view of the spatio-temporal distribution of sclerostin, β-catenin and osterix at the tissue to subcellular level in a coherent manner, and uncovered their spatio-temporal cooperation in postnatal bone development, thus providing evidence that they link skeletogenic growth and functional bone development.
Databáze: OpenAIRE
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