An innovative panel containing a set of insertion/deletion loci for individual identification and its forensic efficiency evaluations in Chinese Hui ethnic minority
| Autor: | Tingting Kong, Chong Chen, Xiaoye Jin, Bofeng Zhu, Wenqing Zhang, Yuxin Guo, Hao-Tian Meng, Wei Cui |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Forensic Genetics
0301 basic medicine China lcsh:QH426-470 Genotyping Techniques Population individual identification Population genetics 030105 genetics & heredity Biology Polymorphism Single Nucleotide Sensitivity and Specificity genetic structure analysis Fixation index Loss of heterozygosity 03 medical and health sciences Gene Frequency INDEL Mutation Chinese Hui group Polymorphism (computer science) Genetics Humans Allele Indel education Molecular Biology Genetics (clinical) education.field_of_study Original Articles humanities lcsh:Genetics 030104 developmental biology Genetic Loci Evolutionary biology Biometric Identification Original Article Identification (biology) insertion and deletion polymorphisms |
| Zdroj: | Molecular Genetics & Genomic Medicine Molecular Genetics & Genomic Medicine, Vol 8, Iss 2, Pp n/a-n/a (2020) |
| ISSN: | 2324-9269 |
| Popis: | Background Individual identification is one of the most important tasks in the field of forensic genetics. Insertion/Deletion (InDel) polymorphism marker has been a promising marker for individual identification. However, a part of InDel loci in commonly used commercial kit show low polymorphisms in Chinese populations. Methods We evaluated a panel of 35 InDel loci constructed previously for individual identifications in Hui group. Subsequently, population data of three Chinese populations from 1,000 Genomes Project database were used to evaluate individual identification performance of these 35 InDels. Forensic parameters, such as heterozygosity, power of exclusion, match probability and power of discrimination, were calculated to evaluate the forensic efficiency of these loci in Hui group. The heatmap of insertion allelic frequencies, Nei's genetic distances, pairwise fixation index values, principal component analyses and admixture analyses were used to analyze the genetic differentiations and structure between Hui group and other populations. Results In studied Hui group, besides rs3054057, polymorphism information content values of the remaining loci were greater than 0.3. Values of expected heterozygosity of these loci were close to 0.5. The combined power of discrimination and power of exclusion values were 0.99999999999999659609 and 0.998682, respectively. Analyses of population genetics revealed that Chinese Hui group had closer genetic relationships with East Asian populations than other intercontinental populations. Conclusion The forensic statistical analyses revealed these loci showed relatively high genetic polymorphisms in Chinese Hui group, and could be served as a useful tool for individual identifications in Hui group. Population genetic evaluations indicated that Chinese Hui group had close genetic relationships with East Asian populations. We previously constructed a panel of 35 InDel loci with relative high polymorphisms in Chinese three populations for individual identifications. In present study, the forensic statistical analyses also revealed these loci showed relatively high genetic polymorphisms in studied Chinese Hui group and could be served as a tool in the aspect of individual identifications in Chinese Hui group. Population genetic evaluations indicated that Chinese Hui group had close genetic relationships with East Asian populations. |
| Databáze: | OpenAIRE |
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