Induction of Male Sterility in Tobacco by Anther-Specific Expression of the Gene for Ricin Enzymatic Subunit A Chain RTA
| Autor: | Chang-Bo Dai, Yu-He Sun, Jing Lv |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Plant Infertility Sterility Nicotiana tabacum Protein subunit Transgene Stamen Bioengineering Flowers Ricin Biology 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Tobacco Gene expression Molecular Biology Gene 030304 developmental biology 0303 health sciences fungi food and beverages Plants Genetically Modified biology.organism_classification Cell biology chemistry Biotechnology |
| Zdroj: | Molecular Biotechnology. 62:364-369 |
| ISSN: | 1559-0305 1073-6085 |
| DOI: | 10.1007/s12033-020-00252-0 |
| Popis: | Targeted gene expression in plants allows us to further study biological traits of interest, such as reproductive and developmental processes. Here, the tobacco TA29 anther-specific promoter was used to direct the expression of the ricin enzymatic subunit A (RTA) in transgenic tobacco plants, phenotypic analysis of the resulting positive transgenic tobacco (Nicotiana tabacum L.) plants demonstrated that RTA expression led to a reduction in pistil length and shriveling of anthers, as well as the grayish-brown color of anthers, the reduced pollen viability and male sterility. For the first time, a plant-derived ricin gene enzymatic subunit A (RTA) expression system under the tissue-specific promoter was demonstrated to be sensitive and efficient in controlling plant sterility and creating male-sterile materials. Consequently, it could be used to control other agronomic traits and produce hybrid seeds in plants in the future. |
| Databáze: | OpenAIRE |
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