Inhibition of GSK-3β enhances neural differentiation in unrestricted somatic stem cells
| Autor: | Marzieh Mowlavi Vardanjani, Fatemeh Vahid Dastjerdi, Mahin Sadat Chavoshi, Masoud Soleimani, Amir Atashi, Anahita Shahraz, Irandokht Khaki Najafabadi, Bahman Zeynali, Azita Parvaneh Tafreshi |
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| Rok vydání: | 2012 |
| Předmět: |
Indoles
Time Factors Somatic cell Neurogenesis Blotting Western Active Transport Cell Nucleus Tretinoin Biology Glycogen Synthase Kinase 3 Tubulin 1-Methyl-3-isobutylxanthine Oximes Humans Wnt Signaling Pathway Cells Cultured beta Catenin Cell Nucleus Glycogen Synthase Kinase 3 beta Stem Cells Wnt signaling pathway Cell Biology General Medicine Fetal Blood Embryonic stem cell Immunohistochemistry Hedgehog signaling pathway Neural stem cell Cell biology Stem cell Adult stem cell |
| Zdroj: | Cell biology international. 36(11) |
| ISSN: | 1095-8355 |
| Popis: | GSK-3β is a key molecule in several signalling pathways, including the Wnt/β-catenin signalling pathway. There is increasing evidence suggesting Wnt/β-catenin signalling is involved in the neural differentiation of embryonic, somatic and neural stem cells. However, a large body of evidence indicates that this pathway maintains stem cells in a proliferative state. To address this controversy, we have investigated whether the Wnt/β-catenin pathway is present and involved in the neural differentiation of newly introduced USSCs (unrestricted somatic stem cells). Our results indicate that the components of Wnt/β-catenin signalling are present in undifferentiated USSCs. We also show that the treatment of neurally induced USSCs with BIO (6-bromoindirubin-3'-oxime), a specific GSK-3β inhibitor and Wnt activator, for 5 and 10 days results in increased expression of a general neuronal marker (β-tubulin III). Moreover, the expression of pGSK-3β and stabilized β-catenin increased by BIO in neurally induced USSCs, indicates that the Wnt pathway is activated and functional in these cells. Thus, inhibition of GSK-3β in USSCs enhances their neural differentiation, which suggests a positive role of the Wnt/β-catenin signalling pathway towards neural fate. |
| Databáze: | OpenAIRE |
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