Remote Fingerstick Blood Collection for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antibody Testing
| Autor: | Jochen K. Lennerz, Tyler E. Miller, Andrea L. Nixon, Mandakolathur R. Murali, Lisa M Mahanta, Julia Thierauf, Michael G Astudillo, Grace Kirkpatrick, Diane Yang, Anand S. Dighe, Wilfredo F. Garcia-Beltran, Vivek Naranbhai, A. John Iafrate |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Emergency Use Authorization Fingerstick 030106 microbiology Antibodies Viral Roche Diagnostics Sensitivity and Specificity 01 natural sciences COVID-19 Serological Testing Pathology and Forensic Medicine Serology Fingers 03 medical and health sciences Seroepidemiologic Studies High-Throughput Screening Assays Humans Medicine Seroprevalence Pandemics Blood Specimen Collection biology SARS-CoV-2 business.industry 010401 analytical chemistry COVID-19 General Medicine Virology 0104 chemical sciences Medical Laboratory Technology Remote Sensing Technology biology.protein Antibody business |
| Zdroj: | Archives of Pathology & Laboratory Medicine. 145:415-418 |
| ISSN: | 1543-2165 0003-9985 |
| DOI: | 10.5858/arpa.2020-0713-sa |
| Popis: | The rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has propelled the rapid development of serologic tests that can detect anti–SARS-CoV-2 antibodies. These have been used for studying the prevalence and spread of infection in different populations, and helping establish a recent diagnosis of coronavirus disease 2019 (COVID-19), and will likely be used to confirm humoral immunity after infection or vaccination. However, nearly all lab-based high-throughput SARS-CoV-2 serologic assays require a serum sample from venous blood draw, limiting their applications and scalability. Here, we present a method that enables large-scale SARS-CoV-2 serologic studies by combining self or office collection of fingerprick blood with a volumetric absorptive microsampling device (Mitra, Neoteryx LLC) with a high-throughput electrochemiluminescence-based SARS-CoV-2 total antibody assay (Roche Elecsys, Roche Diagnostics Inc) that is emergency use authorization approved for use on serum samples and widely used by clinical laboratories around the world. We found that the Roche Elecsys assay has a high dynamic range that allows for accurate detection of SARS-CoV-2 antibodies in serum samples diluted 1:20 as well as contrived dried blood extracts. Extracts of dried blood from Mitra devices acquired in a community seroprevalence study showed near identical sensitivity and specificity in detection of SARS-CoV-2 antibodies compared with neat sera using predefined thresholds for each specimen type. Overall, this study affirms the use of Mitra dried blood collection device with the Roche Elecsys SARS-CoV-2 total antibody assay for remote or at-home testing as well as large-scale community seroprevalence studies. |
| Databáze: | OpenAIRE |
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