Epithelial expression and role of secreted STC1 on asthma airway hyperresponsiveness through calcium channel modulation

Autor: Yunhui Wu, Xin Yao, Man Jia, Ian M. Adcock, Xiaoyi Yan, Yi Yang, Jie Jiang, Yingwei Ou, Mao Huang, Yaqi Meng, Jiayan Xu
Přispěvatelé: Engineering & Physical Science Research Council (EPSRC), Medical Research Council (MRC)
Rok vydání: 2021
Předmět:
0301 basic medicine
Allergy
Contraction (grammar)
0302 clinical medicine
Immunology and Allergy
Mice
Inbred BALB C
biology
medicine.diagnostic_test
SMOOTH-MUSCLE
INHIBITOR
respiratory system
1107 Immunology
Interleukin 13
medicine.symptom
epithelium- derived relaxing factor
Bronchoalveolar Lavage Fluid
Life Sciences & Biomedicine
medicine.medical_specialty
Ovalbumin
Immunology
Bronchi
Inflammation
03 medical and health sciences
INFLAMMATION
Internal medicine
Respiratory Hypersensitivity
medicine
Animals
Humans
Glycoproteins
Asthma
INTERLEUKIN-13
Science & Technology
business.industry
airway hyperresponsiveness
asthma
medicine.disease
respiratory tract diseases
stanniocalcin-1
MICE
030104 developmental biology
Endocrinology
Bronchoalveolar lavage
030228 respiratory system
CELLS
biology.protein
calcium channel
Calcium Channels
business
calcium channel；epithelium- derived relaxing factor
Ex vivo
RESPONSES
Zdroj: Allergy. 76:2475-2487
ISSN: 1398-9995
0105-4538
DOI: 10.1111/all.14727
Popis: BACKGROUND Asthma is characterized by airway hyperresponsiveness (AHR), inflammation, and airway remodeling. Airway hyperresponsiveness results from enhanced airway smooth muscle (ASM) contraction potentially under the control of an epithelium-derived relaxing factor (EpDRF). However, relatively rare is known about EpDRF. We aimed to elucidate the role of epithelium-derived stanniocalcin-1 (STC1) on AHR and ASM contraction. METHODS Stanniocalcin-1 levels in the serum of asthmatic patients and healthy volunteers and in bronchoalveolar lavage fluid (BALF) from ovalbumin (OVA)-challenged mice were measured by ELISA. The effects of exogenous STC1 on AHR and on inflammation were examined in mice. IL-13 modulation of STC1 mRNA and protein levels was studied in human bronchial epithelial cell lines (16HBE). The function of STC1 on Ca2+ influx and ASM contraction was examined ex vivo. RESULTS Serum STC1 was decreased in asthma (n = 93) compared with healthy volunteers (1071 ± 30.4 vs 1414 ± 75.1 pg/ml, p
Databáze: OpenAIRE
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