Combinational therapy of lithium and human neural stem cells in rat spinal cord contusion model
| Autor: | Sahar Kiani, Masoomeh Zareikheirabadi, Razieh Jaberi, Zahra Naghdabadi, Atiyeh Mohammadshirazi, Hoda Sadrosadat, Hossein Baharvand, Sara Mirsadeghi, Mahdieh Ghaneezabadi, Mehdi Fardmanesh |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male Lithium (medication) Physiology Cell Survival Clinical Biochemistry Pharmacology Lithium Motor Activity 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Neural Stem Cells Cell Movement Medicine Animals Humans Evoked potential Rats Wistar Spinal cord injury Spinal Cord Injuries Wound Healing Behavior Animal business.industry Macrophages Cell Differentiation Cell Biology medicine.disease Spinal cord Evoked Potentials Motor Combined Modality Therapy Neural stem cell Transplantation Disease Models Animal 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Lithium chloride Stem cell business medicine.drug Stem Cell Transplantation |
| Zdroj: | Journal of cellular physiology. 234(11) |
| ISSN: | 1097-4652 |
| Popis: | A large number of treatment approaches have been used for spinal cord injury improvement, a medically incurable disorder, and subsequently stem cell transplantation appears to be a promising strategy. The main objective of this study is to ascertain whether combinational therapy of human neural stem cells (hNSCs) together with lithium chloride improves cell survival, proliferation, and differentiation in a rat spinal contusion model, or not. Contusive spinal cord injury was implemented on Wistar male rats. Experimental groups comprised of: control, hNSCs transplanted, lithium chloride (Li), and hNSCs and lithium chloride (hNSCs + Li). In every experimental group, locomotor activity score and motor evoked potential (MEP) were performed to evaluate motor recovery as well as histological assessments to determine mechanisms of improvement. In accordance with our results, the hNSCs + Li and the Li groups showed significant improvement in locomotor scores and MEP. Also, Histological assessments revealed that transplanted hNSCs are capable of differentiation and migration along the spinal cord. Although NESTIN-positive cells were proliferated significantly in the Lithium group in comparison with control and the hNSCs + Li groups, the quantity of ED1 cells in the hNSCs + Li was significantly larger than the other two groups. Our results demonstrate that combinational therapy of hNSCs with lithium chloride and lithium chloride individually are adequate for ameliorating more than partial functional recovery and endogenous repair in spinal cord-injured rats. |
| Databáze: | OpenAIRE |
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