Functional and sequence analysis of human neuroglobin gene promoter region
| Autor: | Sjaak Philipsen, Lydia Y.L. Cheng, Sha Sha, Zhipeng Tian, Kian Cheng Tan-Un, Wei Zhang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Chromatin Immunoprecipitation
5' Flanking Region Sp1 Transcription Factor Molecular Sequence Data Biophysics Neuroglobin Electrophoretic Mobility Shift Assay Nerve Tissue Proteins Biology Regulatory Sequences Nucleic Acid Decitabine Biochemistry Cell Line Structural Biology Transcription (biology) Cell Line Tumor Genetics Humans Globin Enzyme Inhibitors Luciferases Promoter Regions Genetic Molecular Biology Gene Sp1 transcription factor Binding Sites Base Sequence Reverse Transcriptase Polymerase Chain Reaction Promoter Methylation Molecular biology Globins Sp3 Transcription Factor Gene Expression Regulation DNA methylation Azacitidine Mutagenesis Site-Directed Transcription Initiation Site HeLa Cells Protein Binding |
| Zdroj: | Biochimica et biophysica acta. 1809(4-6) |
| ISSN: | 0006-3002 |
| Popis: | Neuroglobin (Ngb), a recently found oxygen-binding protein belonging to the vertebrate globin family, is mainly expressed in neurons of brains and eyes. Current studies have revealed diverse potential functions of Ngb and it was found to be able to reduce the severity of stroke and Alzheimer's disease, implying its importance in brains. However, the mechanism of Ngb regulation of transcription has not been elucidated yet. In this study, we analyzed the 5′-flanking region of human neuroglobin gene ( NGB ) and identified a transcription start site (TSS) located at − 306 bp relative to the translation start site ATG. We characterized the proximal promoter of NGB and found two GC-boxes located at − 16 and + 30 bp relative to the TSS which are bound by transcription factor Sp1 and Sp3. Mutation of either GC-box led to a significant reduction in NGB promoter activity, while overexpression of Sp1 and Sp3 resulted in activation of the promoter. However, two putative NRSE sites (− 359 and − 127 bp relative to the TSS) apparently showed no influence on NGB tissue-specific expression. Treatment of two non-neuronal cell lines HeLa and BEAS-2B with 5-aza-2′-deoxycytidine remarkably induced NGB expression, suggesting a potential role of DNA methylation in regulating NGB tissue-specific expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|