Low prevalence of antifungal resistant Candida africana, in the C. albicans complex causing vulvovaginal candidiasis
| Autor: | Samira Izadi, Mahtab Ashrafi-Khozani, Zahra Ghahri-Mobaser, Saham Ansari, Azam Fattahi, Elham Razmjou, Maryam Roudbary, Maryam Rahimi, Shirin Farahyar, Mehraban Falahati |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Itraconazole Women's health Microbial genomics Mycology Biology Microbiology law.invention 03 medical and health sciences 0302 clinical medicine law medicine DNA sequencing lcsh:Social sciences (General) lcsh:Science (General) Polymerase chain reaction Vaginitis Multidisciplinary Clotrimazole Broth microdilution medicine.disease biology.organism_classification Corpus albicans 030104 developmental biology Antimicrobial lcsh:H1-99 030217 neurology & neurosurgery Fluconazole Candida dubliniensis lcsh:Q1-390 medicine.drug |
| Zdroj: | Heliyon, Vol 6, Iss 3, Pp e03619-(2020) |
| ISSN: | 2405-8440 |
| DOI: | 10.1016/j.heliyon.2020.e03619 |
| Popis: | The Candida (C.) albicans complex includes C. albicans, C. dubliniensis, C. stellatoidea, and C. africana, with the last mentioned as an important emerging agent of vulvovaginal candidiasis (VVC). The aim of the study was to identify C. africana and C. dubliniensis and assess their drug susceptibility in vaginitis. One-hundred Candida isolates of the C. albicans complex from women diagnosed with vaginitis and from vaginal samples in the culture collection of a medical mycology laboratory were examined. Species of the C. albicans complex were identified with conventional and molecular methods using polymerase chain reaction (PCR) for amplification and sequencing of the internal transcribed spacer (ITS) region, PCR for partial amplification of hyphal wall protein 1 (HWP1) gene and duplex PCR. The effects of antifungal drugs were evaluated according to standard broth microdilution protocols. Ninety-seven C. albicans (97%) and three C. africana (3%) isolates were identified. Results of susceptibility testing revealed one isolate of C. africana to be resistant to both clotrimazole and fluconazole, and one showed reduced susceptibility to itraconazole. Identification of Candida species especially C. africana in vaginitis is crucial, there are varying levels of resistance to antifungal drugs. |
| Databáze: | OpenAIRE |
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