Chemical Mapping of Leishmania Infection in Live Cells by SERS Microscopy
| Autor: | Michael Laue, Daniela Drescher, Janina Kneipp, Geo Semini, Vesna Živanović, Toni Aebischer |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival Endosome Metal Nanoparticles 02 engineering and technology Vacuole Spectrum Analysis Raman Analytical Chemistry Mice 03 medical and health sciences chemistry.chemical_compound Microscopy Animals Parasite hosting Amastigote Leishmania Ergosterol biology Macrophages 021001 nanoscience & nanotechnology biology.organism_classification In vitro 030104 developmental biology chemistry Biophysics Gold 0210 nano-technology |
| Zdroj: | Analytical Chemistry. 90:8154-8161 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.8b01451 |
| Popis: | We report the direct probing of the molecular composition of Leishmania-infected macrophage cells in vitro by surface-enhanced Raman scattering (SERS). The microscopic mapping data indicate local abundance and distribution of molecular species that are very characteristic of the infection and that are observed here simultaneously. As revealed by electron microscopy, the gold nanoprobes used for SERS microspectrosopy have access to the parasitophorous vacuoles (PV) through the endosomal system. SERS nanoprobes located in the direct proximity to the parasite, in the greater volume of the PV, and in endolysosomal compartments in other cellular regions, respectively, report a characteristic chemical composition for each respective location. The data enable assessment of the distribution of ergosterol and cholesterol in the amastigote stage of the parasite and its immediate surroundings in the vacuole. Proteophosphoglycans of parasite origin, an important hallmark of the infection, are identified throughout the PV. |
| Databáze: | OpenAIRE |
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