Serodiagnosis of hepatitis C virus (HCV) infection with an HCV core protein molecularly expressed by a recombinant baculovirus
| Autor: | Hiroyoshi Ohba, Joe Chiba, Tatsuo Miyamura, Shu Kikuchi, Tohru Katayama, Yoshiharu Matsuura, Yushiro Watanabe, Izumu Saito |
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| Rok vydání: | 1991 |
| Předmět: |
Insecta
Hepatitis C virus Genetic Vectors Blood Donors Enzyme-Linked Immunosorbent Assay Transfection medicine.disease_cause Cell Line Serology Capsid Antigen medicine Animals Humans Blood Transfusion Serologic Tests Hepatitis Antibodies Mass screening Hepatitis Multidisciplinary biology Viral Core Proteins Hepatitis C medicine.disease Virology Immunology biology.protein Hepatitis C Antigens Antibody Viral hepatitis Baculoviridae Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 88:4641-4645 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | An enzyme-linked immunosorbent assay (ELISA) was developed for serological diagnosis of hepatitis C virus (HCV) infection, using HCV core protein (p22) synthesized by a recombinant baculovirus. Among 58 clinically well-defined chronic non-A, non-B hepatitis (NANBH) patients, 49 (84.5%) were positive for p22 antibody (anti-p22), whereas 42 (72.4%) were positive for C100-3 antibody (anti-C100-3), as measured by the present assay using the HCV nonstructural protein as antigen. Thirty-nine patients (67.2%) had both antibodies. No significant level of anti-p22 was detected in sera of chronic hepatitis B patients or normal blood donors. In typical post-transfusion NANBH patients, anti-p22 could be detected at, or even before, the first alanine aminotransferase peak. Anti-p22 was also detected in blood donors who were previously shown to be involved in transmitting HCV but in whose serum anti-C100-3 was not detectable. The ELISA detecting antibody to the HCV core protein expressed and properly processed in animal cells will be useful for mass screening of donor blood as well as for early diagnosis of hepatitis C. |
| Databáze: | OpenAIRE |
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