An integrated, stacked microlaboratory for biological agent detection with DNA and immunoassays
| Autor: | Robert W. Haigis, Bob Martinsons, Donald S. Thomas, Ying Huang, Paul D. Swanson, Janice Bell, Anita H. Forster, Marcus Tirado, Michael Krihak, Joon Mo Yang, R. Bruce Wallace |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Electrophoresis Materials science Biomedical Engineering Biophysics Nanotechnology Biosensing Techniques Shiga Toxin 1 medicine.disease_cause Enterotoxins chemistry.chemical_compound Electromagnetic Fields Biological Warfare Escherichia coli Electrochemistry medicine Fluidics Immunoassay Miniaturization Chromatography medicine.diagnostic_test DNA–DNA hybridization Multiple displacement amplification Nucleic Acid Hybridization Equipment Design General Medicine Semiconductors chemistry DNA Probes Nucleic Acid Amplification Techniques Layer (electronics) Polyimide DNA Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 17:605-618 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/s0956-5663(02)00023-4 |
| Popis: | An integrated, stacked microlaboratory for performing automated electric-field-driven immunoassays and DNA hybridization assays was developed. The stacked microlaboratory was fabricated by orderly laminating several different functional layers (all 76 x 76 mm(2)) including a patterned polyimide layer with a flip-chip bonded CMOS chip, a pressure sensitive acrylic adhesive (PSA) layer with a fluidic cutout, an optically transparent polymethyl methacrylate (PMMA) film, a PSA layer with a via, a patterned polyimide layer with a flip-chip bonded silicon chip, a PSA layer with a fluidic cutout, and a glass cover plate layer. Versatility of the stacked microlaboratory was demonstrated by various automated assays. Escherichia coli bacteria and Alexa-labeled protein toxin staphylococcal enterotoxin B (SEB) were detected by electric-field-driven immunoassays on a single chip with a specific-to-nonspecific signal ratios of 4.2:1 and 3.0:1, respectively. Furthermore, by integrating the microlaboratory with a module for strand displacement amplification (SDA), the identification of the Shiga-like toxin gene (SLT1) from E. coli was accomplished within 2.5 h starting from a dielectrophoretic concentration of intact E. coli bacteria and finishing with an electric-field-driven DNA hybridization assay, detected by fluorescently labeled DNA reporter probes. The integrated microlaboratory can be potentially used in a wide range of applications including detection of bacteria and biowarfare agents, and genetic identification. |
| Databáze: | OpenAIRE |
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