New Genes Implicated in the Protection of Anaerobically Grown Escherichia coli against Nitric Oxide
Autor: | Lígia M. Saraiva, Marta C. Justino, Miguel Teixeira, João B. Vicente |
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Rok vydání: | 2005 |
Předmět: |
Hemeproteins
Time Factors DNA Repair Transcription Genetic Molecular Sequence Data Inflammation Biology Nitric Oxide medicine.disease_cause Biochemistry Nitric oxide Gene product chemistry.chemical_compound Bacterial Proteins Escherichia coli medicine Transcriptional regulation Amino Acid Sequence Molecular Biology Gene Oligonucleotide Array Sequence Analysis chemistry.chemical_classification Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Escherichia coli Proteins Macrophages DNA Cell Biology Enzyme chemistry Mutation RNA medicine.symptom Anaerobic exercise Transcription Factors |
Zdroj: | Journal of Biological Chemistry. 280:2636-2643 |
ISSN: | 0021-9258 |
Popis: | Nitric oxide produced by activated macrophages plays a key role as one of the immune system's weapons against pathogens. Because the lifetime of nitric oxide is short in aerobic conditions, whereas in anaerobic conditions the cytotoxic effects of nitric oxide are greatly increased as in the infection/inflammation processes, it is important to establish which systems are able to detoxify nitric oxide under anaerobic conditions. In the present work a new set of Escherichia coli K-12 genes conferring anaerobic resistance to nitric oxide is presented, namely the gene product of YtfE and a potential transcriptional regulator of the helix-turn-helix LysR-type (YidZ). The crucial role of flavohemoglobin for anaerobic nitric oxide protection is also demonstrated. Furthermore, nitric oxide is shown to cause a significant alteration of the global E. coli gene transcription profile that includes the increase of the transcript level of genes encoding for detoxification enzymes, iron-sulfur cluster assembly systems, DNA-repairing enzymes, and stress response regulators. |
Databáze: | OpenAIRE |
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