Expression pattern of Bombyx vasa-like (BmVLG) protein and its implications in germ cell development
| Autor: | Jae Min Lee, Hajime Nakao, Toshio Kanda, Masami Shimoda, Masatsugu Hatakeyama |
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| Rok vydání: | 2005 |
| Předmět: |
Genetics
endocrine system Embryo Nonmammalian urogenital system fungi Embryo Biology Bombyx biology.organism_classification Embryonic stem cell Cell biology Germ Cells medicine.anatomical_structure medicine Animals Insect Proteins Female Germ line development Cellularization Developmental biology Germ cell Developmental Biology Germ plasm |
| Zdroj: | Development Genes and Evolution. 216:94-99 |
| ISSN: | 1432-041X 0949-944X |
| DOI: | 10.1007/s00427-005-0033-8 |
| Popis: | Germ cell development in the silkworm Bombyx mori is interesting in that the species has no recognizable germ plasm, and its germ cells appear first on the ventral side of the embryo, not on the posterior pole as in Drosophila melanogaster. We previously reported the isolation of a vasa homologue (BmVLG) from B. mori and revealed the specific expression of transcript in the germ cells. In this paper, we describe the embryonic expression pattern of BmVLG protein. Consistent with the lack of recognizable germ plasm, the protein is not localized in freshly laid eggs, and its specific expression is first detectable several hours after energids penetrate the periplasm. This is in contrast to D. melanogaster, where germ cell lineage can be traced with anti-vasa antibody just after the formation of pole cells as they sequester vasa-positive germ (pole) plasm during cellularization. It is also revealed that, within the first few hours of their appearance when extensive cell movement does not seem to occur, stained cells are sometimes widely dispersed along the midline, which eventually may lead to the formation of ectopic germ cells. The implications of these results for germ cell development are discussed. |
| Databáze: | OpenAIRE |
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