Time-dependent Expression of MMP-2 and TIMP-2 after Rats Skeletal Muscle Contusion and Their Application to Determine Wound Age
| Autor: | Rui Zhao, Da-Wei Guan, Zhen-Hua Zhang, Yan Zhang, Tian-Shui Yu, Zhuang Li |
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| Rok vydání: | 2014 |
| Předmět: |
0301 basic medicine
Male Forensic pathology Pathology medicine.medical_specialty Time Factors Contusions Matrix metalloproteinase Real-Time Polymerase Chain Reaction Pathology and Forensic Medicine Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Genetics Medicine Macrophage Animals 030216 legal & forensic medicine RNA Messenger Muscle Skeletal Forensic Pathology Messenger RNA Tissue Inhibitor of Metalloproteinase-2 business.industry Macrophages Colocalization Skeletal muscle Relative Quantity 030104 developmental biology medicine.anatomical_structure Mrna level Models Animal Matrix Metalloproteinase 2 business Biomarkers |
| Zdroj: | Journal of forensic sciences. 61(2) |
| ISSN: | 1556-4029 |
| Popis: | The ability to determine vitality and estimate the survival period after a wound is critical in routine forensic practice. The mRNA levels of MMP-2 and TIMP-2 were examined using quantitative real-time RT-PCR to determine the age of a wound. Furthermore, the colocalization of them with Macrophage Marker, respectively, was detected by double immunofluorescence, and a standardized rat model of skeletal muscle contusion was established. In the antemortem contused groups, a large number of macrophages showed positive staining for MMP-2 and TIMP-2, and the expression of MMP-2 and TIMP-2 mRNA increased sharply at 3 days postinjury, with relative quantities of 5.75 and 2.98. No samples in the other groups showed relative quantities of >5.75 and 2.98; therefore, relative quantities exceeding 5.75 and 2.98 were strongly indicated 3 days after contusion. In addition, there was a significant decrease in the relative quantity in the postmortem contused groups, indicating that they were useful for diagnosing vitality. |
| Databáze: | OpenAIRE |
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