Short-term High-fat Overfeeding Does Not Induce NF-κB Inflammatory Signaling in Subcutaneous White Adipose Tissue
| Autor: | Nicolette C. Bishop, Carl J. Hulston, Alexander J. Wadley, Rachel M Woods, Rebecca Dewhurst-Trigg, Lauren B. Sherar, Oonagh Markey |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Blood Glucose Male 0301 basic medicine medicine.medical_specialty Adipose Tissue White Endocrinology Diabetes and Metabolism medicine.medical_treatment Clinical Biochemistry Subcutaneous Fat 030209 endocrinology & metabolism Context (language use) Inflammation White adipose tissue Diet High-Fat Biochemistry Eating 03 medical and health sciences Overnutrition 0302 clinical medicine Endocrinology Internal medicine medicine Humans Insulin Phosphorylation medicine.diagnostic_test business.industry Biochemistry (medical) NF-kappa B medicine.disease Cholesterol 030104 developmental biology Female Tumor necrosis factor alpha Insulin Resistance medicine.symptom business Lipid profile Weight gain Signal Transduction |
| Zdroj: | The Journal of Clinical Endocrinology & Metabolism. 105:2162-2176 |
| ISSN: | 1945-7197 0021-972X |
| DOI: | 10.1210/clinem/dgaa158 |
| Popis: | Context It is unclear how white adipose tissue (WAT) inflammatory signaling proteins respond during the early stages of overnutrition. Objective To investigate the effect of short-term, high-fat overfeeding on fasting abdominal subcutaneous WAT total content and phosphorylation of proteins involved in nuclear factor-κB (NF-κB) inflammatory signaling, systemic metabolic and inflammatory biomarkers. Design Individuals consumed a high-fat (65% total energy from total fat), high-energy (50% above estimated energy requirements) diet for 7 days. Results Fifteen participants (aged 27 ± 1 years; body mass index 24.4 ± 0.6 kg/m2) completed the study. Body mass increased following high-fat overfeeding (+1.2 ± 0.2 kg; P < 0.0001). However, total content and phosphorylation of proteins involved in NF-κB inflammatory signaling were unchanged following the intervention. Fasting serum glucose (+0.2 ± 0.0 mmol/L), total cholesterol (+0.4 ± 0.1 mmol/L), low-density lipoprotein cholesterol (+0.3 ± 0.1 mmol/L), high-density lipoprotein cholesterol (+0.2 ± 0.0 mmol/L), and lipopolysaccharide-binding protein (LBP; +4.7 ± 2.1 µg/mL) increased, whereas triacylglycerol concentrations (−0.2 ± 0.1 mmol/L) decreased following overfeeding (all P < 0.05). Systemic biomarkers (insulin, soluble cluster of differentiation 14 [CD14], C-reactive protein, interleukin-6, tumor necrosis factor-α and monocyte chemoattractant protein-1) and the proportion and concentration of circulating CD14+ monocytes were unaffected by overfeeding. Conclusion Acute lipid oversupply did not impact on total content or phosphorylation of proteins involved in WAT NF-κB inflammatory signaling, despite modest weight gain and metabolic alterations. Systemic LBP, which is implicated in the progression of low-grade inflammation during the development of obesity, increased in response to a 7-day high-fat overfeeding period. |
| Databáze: | OpenAIRE |
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