In-depth characterization of monocyte subsets during the course of healthy pregnancy
| Autor: | Cai Niklaas Feldmann, Caroline Pflitsch, Virginija Jazbutyte, Laura Richert, Kurt Hecher, Susanne Ziegler, Marcus Altfeld, Sven Hendrik Hagen, J. Goletzke, Petra C. Arck, Anke Diemert, Thomas Renné |
|---|---|
| Přispěvatelé: | Heinrich Pette Institute [Hamburg], Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), Statistics In System biology and Translational Medicine (SISTM), Inria Bordeaux - Sud-Ouest, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)- Bordeaux population health (BPH), Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bordeaux (UB)-Institut de Santé Publique, d'Épidémiologie et de Développement (ISPED)-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Bordeaux [Bordeaux], Universitaetsklinikum Hamburg-Eppendorf = University Medical Center Hamburg-Eppendorf [Hamburg] (UKE), C.P., C.N.F. S.H.H. and S.M.Z. received funding from the German Research Foundation (DFG) through the Clinical Research Unit(CRU)296 and the Collaborative Research Centre (CRC) 841 and from the Landesforschungsförderung Hamburg LFF-FV 45 Geschlechtsdimorphismus im Immunsystem. This work has been funded in part by the Pathogenesis and the Viral Latency Programs of the Heinrich Pette Institute, Leibniz Institute for Experimental Virology. This work was supported by the |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Adult
0301 basic medicine CCR2 Receptors CCR2 Pregnancy-related hormones Immunology Population Peripheral blood mononuclear cell Monocytes Immunophenotyping Young Adult 03 medical and health sciences 0302 clinical medicine Immune system Pregnancy medicine Humans Immunology and Allergy Chorionic Gonadotropin beta Subunit Human Prospective Studies education education.field_of_study CD11b Antigen 030219 obstetrics & reproductive medicine Innate immune system business.industry Monocyte Intracellular cytokines Obstetrics and Gynecology medicine.disease Immunity Innate 3. Good health SISTM 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Receptors Granulocyte-Macrophage Colony-Stimulating Factor [SDV.IMM]Life Sciences [q-bio]/Immunology Female Pregnancy Trimesters business Monocyte subsets |
| Zdroj: | Journal of Reproductive Immunology Journal of Reproductive Immunology, Elsevier, 2020, 141, pp.103151. ⟨10.1016/j.jri.2020.103151⟩ Journal of Reproductive Immunology, 2020, 141, pp.103151. ⟨10.1016/j.jri.2020.103151⟩ |
| ISSN: | 0165-0378 |
| DOI: | 10.1016/j.jri.2020.103151⟩ |
| Popis: | International audience; Pregnancy represents an immunological challenge for the maternal immune system. Pregnancy augments innate immune responses, and particularly monocytes contribute to maintaining the balance between pro- and anti-inflammatory immune responses required for the successful sequence of distinct immunological phases throughout pregnancy. Nonetheless, studies that focus on the heterogeneity of monocytes and analyze the alteration of monocyte subsets in a longitudinal approach throughout healthy pregnancies have remained scarce. In this study, we characterized the gradual phenotypic changes of monocyte subsets and the secretory potential of bulk monocytes in peripheral blood mononuclear cells of healthy pregnant women from a population-based prospective birth cohort study. Blood samples at predefined time points were analyzed using flow cytometry for in-depth characterization of monocyte subsets, which confirmed a shift from classical towards intermediate monocytes throughout pregnancy. Principal component analysis revealed characteristic phenotypic changes on monocyte subsets, especially on the intermediate monocyte subset, throughout pregnancy. Pregnancy-related hormones were measured in serum and β-human chorionic gonadotropin levels were significantly associated with expression of CD11b, CD116 and CCR2 on monocyte subsets. TLR4 and TLR7/8 stimulation of monocytes furthermore showed reduced polycytokine production towards the end of pregnancy. These data provide a comprehensive overview of phenotypic changes and secretory potential of monocytes in healthy pregnant women and establish a selective contribution of different monocyte subsets to healthy pregnancy. The results from this study therefore build a basis for future comparisons and evaluation of women with adverse pregnancy outcomes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect