Properties of antigenomic hepatitis delta virus ribozyme that consists of three RNA oligomer strands
| Autor: | M. A. Lukin, R. Sh. Beabealashvilli, Alekseenkova Va, Belianko Ti, L. P. Savochkina |
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| Rok vydání: | 2003 |
| Předmět: |
Stereochemistry
Kinetics Genome Viral Biochemistry Oligomer Catalysis chemistry.chemical_compound Structure-Activity Relationship Nucleotide Magnesium RNA Antisense RNA Catalytic Ternary complex chemistry.chemical_classification biology Base Sequence Molecular Structure Ribozyme General Medicine Dissociation constant chemistry biology.protein Mutagenesis Site-Directed Nucleic Acid Conformation RNA Viral Hepatitis Delta Virus Ternary operation Derivative (chemistry) |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0006-2979 |
| Popis: | A three-strand ribozyme, a derivative of antigenomic hepatitis delta virus (HDV) ribozyme, which consists of subfragments of 16 (L), 17 (S), and 33 nucleotides (B), has been constructed. The ternary B–L–S complex formed by the subfragments in stoichiometric ratio was able to catalyze a self-cleavage reaction. Kinetics of this reaction exhibited biphasic behavior and the same parameters as in the case of natural cis-ribozyme. Study of kinetics of reaction initiated by adding various reaction components and the study of binary complex formation between subfragments B and L, B and S, and also ternary B–L–S complex formation revealed that: 1) in the presence of Mg2+, B and S form a stoichiometric complex, L and S do not form complex at all, while B and L form 2 types of complexes, probably B–L and 2B–L; and addition of S subfragment prevented the formation of the latter complex; 2) the reaction initiated by S subfragment proceeds much slower than that initiated by other components pointing to the possibility that in the absence of S L may form a nonproductive complex with B, which is slowly displaced by S followed by productive ternary complex formation. Dissociation constants for binary B–L, B–S and ternary B–L–S complexes have been estimated. |
| Databáze: | OpenAIRE |
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