Green biosynthesis of silver nanoparticles by Aspergillus niger and its antiamoebic effect against Allovahlkampfia spelaea trophozoite and cyst
| Autor: | Enas Abdelhameed Mahmoud Huseein, Fatma Abdel Aziz Mohamed Mostafa, Haiam Mohamed Mahmoud Farrag, Mona Embarek Mohamed |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Silver 030231 tropical medicine Immunology Metal Nanoparticles Nanoparticle Allovahlkampfia spelaea Silver nanoparticle 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Microscopy Electron Transmission Biosynthesis Spectroscopy Fourier Transform Infrared Humans Trophozoites Fourier transform infrared spectroscopy Keratitis biology Chlorhexidine Aspergillus niger Eukaryota Green Chemistry Technology General Medicine 030108 mycology & parasitology biology.organism_classification Silver nitrate Infectious Diseases chemistry Biochemistry Anti-Infective Agents Local Spectrophotometry Ultraviolet Parasitology HeLa Cells |
| Zdroj: | Experimental Parasitology. 219:108031 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/j.exppara.2020.108031 |
| Popis: | Background Fungi represent an interesting candidate for the synthesis of nanoparticles. The biosynthesis of silver nanoparticles (AgNPs) has many industrial and biomedical indications. We aimed in this work to biologically synthesize silver nanoparticles using Aspergillus niger and to evaluate its effect against the newly identified Allovahlkampfia spelaea that causes resistant human keratitis. Material and methods Aspergillus niger (soil isolate) was treated with silver nitrate to produce silver nanoparticles. AgNPs were characterized by Ultraviolet–Visible Spectroscopy, Transmission Electron Microscopy, and Fourier Transform Infrared Spectroscopy. The effect of the synthesized nanoparticles against Allovahlkampfia spelaea growth, encystation, excystation, and toxicity in host cells was evaluated. Results AgNPs exhibited significant inhibition of Allovahlkampfia spelaea viability and growth of both trophozoites and cysts, with a reduction of amoebic cytotoxic activity in host cells. Conclusion AgNPs may give a promising future to the treatment of Allovahlkampfia spelaea infections in humans. |
| Databáze: | OpenAIRE |
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