Development and Phenotypic Screening of an Ethyl Methane Sulfonate Mutant Population in Soybean
| Autor: | Prakash R. Arelli, Zeinab Yadegari, C. M. Sabbir Ahmed, Ekundayo Adeleke, Ali Taheri, Angelina Bernardini, Vince Pantalone, Mary Jane Espina |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Germplasm TILLING phenotyping food.ingredient late-maturity Linolenic acid Linoleic acid Population Plant Science lcsh:Plant culture Biology 01 natural sciences Soybean oil 03 medical and health sciences chemistry.chemical_compound tilling food lcsh:SB1-1110 Food science soybean education Original Research education.field_of_study EMS fungi food and beverages Methane sulfonate seed compositions Oleic acid 030104 developmental biology chemistry mutation 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science Frontiers in Plant Science, Vol 9 (2018) |
| ISSN: | 1664-462X |
| Popis: | Soybean is an important oil-producing crop in the Fabaceae family and there are increasing demands for soybean oil and other soybean products. Genetic improvement of soybean is needed to increase its production. In order to provide genetic diversity and resources for identifying important genes, a new ethyl methane sulfonate (EMS) mutagenized soybean population was generated using the newly released germplasm, JTN-5203 (maturity group V). Treatment of soybean seeds with 60 mM EMS concentration was found to be suitable for inducing mutation. A total of 1,820 M1 individuals were produced from 15,000 treated seeds. The resulting M2 population was planted in the field for phenotyping. After harvest, seed traits including total oil, protein, starch, moisture content, fatty acid and amino acid compositions were measured by NIR. Phenotypic variations observed in this population include changes in leaf morphology, plant architecture, seed compositions, and yield. Of most interest, we identified plants with increased amounts of total protein (50% vs. 41% for control) and plants with higher amounts of total oil (25% vs. 21.2% control). Similarly, we identified plants with increases in oleic acid content and decreases in linoleic acid and linolenic acid. This EMS mutant population will be used for further studies including screening for various traits such as amino acid pathways, allergens, phytic acids, and other important soybean agronomic traits. In addition, these mutant individuals will be evaluated in the next generation to assess the heritability. Beneficial traits from these mutants can be exploited for future soybean breeding programs. This germplasm can also be used for discovering novel mutant alleles and for functional gene expression analysis using reverse genetics tools such as TILLING. |
| Databáze: | OpenAIRE |
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