LncRNA PTCSC3 affects drug resistance of anaplastic thyroid cancer through STAT3/INO80 pathway

Autor: Meng Jia, Xiubo Lu, Zheng Liu, Zushi Geng, Ling Gu, Xiaoming Wang, Yuxia Fan, Qingling Yuan, Yang Liu
Rok vydání: 2018
Předmět:
STAT3 Transcription Factor
0301 basic medicine
endocrine system
Cancer Research
RNA
Untranslated
endocrine system diseases
Thyroid Gland
Drug resistance
Thyroid Carcinoma
Anaplastic
law.invention
03 medical and health sciences
0302 clinical medicine
law
Cell Line
Tumor
Humans
Medicine
Genes
Tumor Suppressor
Doxorubicin
Thyroid Neoplasms
Anaplastic thyroid cancer
Promoter Regions
Genetic
STAT3
Thyroid cancer
Pharmacology
Antibiotics
Antineoplastic
biology
business.industry
DNA Helicases
medicine.disease
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
030104 developmental biology
Oncology
Drug Resistance
Neoplasm
Chemotherapy Drugs
030220 oncology & carcinogenesis
biology.protein
Cancer research
ATPases Associated with Diverse Cellular Activities
Molecular Medicine
Suppressor
business
Signal Transduction
Research Paper
medicine.drug
Zdroj: Cancer Biology & Therapy. 19:590-597
ISSN: 1555-8576
1538-4047
DOI: 10.1080/15384047.2018.1449610
Popis: Background: LncRNA PTCSC3 is a tumor suppressor in thyroid cancer, and its role in drug resistance of anaplastic thyroid cancer (ATC) to chemotherapy drug doxorubicin was investigated in this study. Methods: Expression of RNA and protein was analyzed by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze the expression rate of CD133(+) cells. The endogenous expression of related genes was modulated by recombinant plasmids and cell transfection. Combination condition and interaction between PTCSC3 and STAT3 were determined by RIP and RNA pull-down assay, respectively. MTT assay was performed to detect cytotoxicity. Chromatin immunoprecipitation was conducted to identify interactions between STAT3 and DNA promoter of INO80. Results: LncRNA PTCSC3 was low-expressed in ATC tissues and cells. Over-expressed PTCSC3 inhibited the drug resistance of ATC to doxorubicin. PTCSC3 negatively regulated STAT3, and STAT3 promoted expression of INO80. PTCSC3 regulated INO80 through STAT3. PTCSC3 suppressed stem cells properties and drug resistance of ATC to doxorubicin. Conclusion: LncRNA PTCSC3 inhibits INO80 expression by negatively regulating STAT3, and thereby attenuating drug resistance of ATC to chemotherapy drug doxorubicin.
Databáze: OpenAIRE
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