Entrapment of Saccharomyces cerevisiae and 3T3 fibroblast cells into blue light cured hydrogels
| Autor: | Swati Mishra, Frank J. Scarano, Paul Calvert |
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| Rok vydání: | 2012 |
| Předmět: |
Sucrose
Materials science Time Factors Light Cell Survival Saccharomyces cerevisiae Biomedical Engineering macromolecular substances Matrix (biology) 3T3 cells Polyethylene Glycols Biomaterials Diffusion Mice Spectroscopy Fourier Transform Infrared medicine Extracellular Animals Fibroblast Prepolymer Microbial Viability Microscopy Confocal biology beta-Fructofuranosidase Cell Membrane technology industry and agriculture Metals and Alloys Hydrogels 3T3 Cells Cells Immobilized Fibroblasts biology.organism_classification Kinetics medicine.anatomical_structure Cross-Linking Reagents Biochemistry Cell culture Self-healing hydrogels Ceramics and Composites Biophysics Protein Binding |
| Zdroj: | Journal of biomedical materials research. Part A. 100(10) |
| ISSN: | 1552-4965 |
| Popis: | Hydrogels, containing yeast cells or fibroblast cells, were fabricated using blue light-induced polymerization technique. The cell-loaded prepolymer formulation was comprised of poly(ethyleneglycol) diacrylate (more than or equal to 50% v/v), 0.5 wt % Eosin Y and 0.5 wt % triethanolamine as the base oligomer, photo-initiator, and co-initiator, respectively. The two model cell lines, Saccharomyces cerevisiae and NIH 3T3 fibroblasts maintained high viability pre- and post-processing. Several bioassays have demonstrated the unaffected intracellular and extracellular activities of the cells entrapped within the hydrogels. Scanning electron microscopy confirmed the proliferation of S. cerevisiae cells that were entrapped and cultivated for 48 h in growth media, which validated the favorable microenvironment and nutrient transport in these gels. Upon entrapment, fibroblast cells remain viable upto 12 h, however they failed to attach within the crosslinked network, thus no further proliferation was observed. The tunable properties of this hydrogel system project it as a useful matrix for specialized biohybrids. |
| Databáze: | OpenAIRE |
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