Effect of betamethasone on the degree of macrophage recruitment and nerve growth factor receptor p75 immunoreaction during recovery of the sciatic nerve after injury: an experimental study in rats
| Autor: | Bo Sunzel, Jan Rosenquist, Yasser Al-Thobaiti, Sture Forsgren, Awwad Al-Bishri |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.drug_class Anti-Inflammatory Agents Betamethasone Receptor Nerve Growth Factor Lesion Double-Blind Method Internal medicine medicine Animals Rats Wistar Glucocorticoids Wound Healing business.industry Macrophages Recovery of Function Ectodysplasins Macrophage Activation Nerve injury Immunohistochemistry Sciatic Nerve Sagittal plane Rats Endocrinology medicine.anatomical_structure Otorhinolaryngology Nerve Growth Factor Receptor p75 Corticosteroid Female Surgery Sciatic nerve Oral Surgery medicine.symptom business Macrophage recruitment medicine.drug |
| Zdroj: | British Journal of Oral and Maxillofacial Surgery. 46:455-459 |
| ISSN: | 0266-4356 |
| DOI: | 10.1016/j.bjoms.2008.01.020 |
| Popis: | This study was designed to explain our previous findings of beneficial effects of betamethasone given perioperatively on decreasing the incidence of neurosensory disturbance after sagittal split osteotomy and improving functional recovery after crush injury to rat sciatic nerves. We analysed the pattern of macrophage recruitment and expression of nerve growth factor p75.The sciatic nerve was crushed in each of 42 animals by tying the nerve against a glass rod for 30s. Half the rats were given betamethasone and half were not. The effect of betamethasone was evaluated immunohistochemically in a double blind manner after 2, 7 and 17 days using antibodies against macrophage marker (ED1) and p75.We found an initial and significant decrease in the number of macrophages recruited after two days in the group treated with betamethasone compared with controls (p=0.001). By 7 days there were significantly more macrophages in the steroid group than in the control group (p=0.001). There was however, a tendency for the number of p75R to be higher in the in the steroid group but the difference was not significant. At 17 days, there were significantly fewer macrophages in the steroid group (p=0.008) than in the control.We conclude that the beneficial effect of a moderate perioperative dose of betamethasone on recovery of a nerve is reflected in the recruitment of macrophages but to only a small extent in expression of p75. |
| Databáze: | OpenAIRE |
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